| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The de novo DNA methyltransferase Dnmt3a regulates gene expression through methylation modification of genomic DNA. DNA methylation of promoters in vertebrates has been reported to function in the regulation of gene expression. However, the role of DNA methylation in the transcription termination region has not yet been reported. In this study, we generated a maternal embryonic knockout mutant (MZdnmt3aa-/-) of the zebrafish dnmt3aa gene, which is a homologue of the mammalian Dnmt3a gene, to elucidate the function of Dnmt3aa in the transcription termination process. We found that DNA hypomethylation in the transcription termination region is involved in the regulation of transcription termination by comprehensive gene expression analysis and whole genome bisulfite sequencing analysis using MZdnmt3aa-/- embryos.
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