| Project/Area Number |
18K06223
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44010:Cell biology-related
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | フォルミン / 細胞間接着 / 細胞質分裂 / 上皮細胞 / タイトジャンクション / アフリカツメガエル / アクチン細胞骨格 / アドレヘンスジャンクション / Rho / アドヘレンスジャンクション / 分裂収縮環 / ライブイメージング |
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| Outline of Final Research Achievements |
Formins are regulated by active Rho GTPases and are involved in the regulation of various cellular processes including cell-cell junction maintenance and contractile ring formation. In this study, I found that two formins Dia1 and Dia2 are localized at tight junctions.But the localization was dispensable for maintenance of cell-cell junction structure and tension generation. Dia3 was localized at contractile rings, but again the localization was dispensable. However, when Dia1 and Dia2 were mislocalized at the contractile ring, we observed increased rate of cytokinesis failure, suggesting that multiple formins under control of the same Rho GTPase function differentially at the junctions and contractile rings and that precise regulation of localization is important for successful cytokinesis.
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| Academic Significance and Societal Importance of the Research Achievements |
細胞接着は日本人が中心的な役割を果たして解明されてきた分野です。本研究は接着部位において細胞骨格を制御する因子について新しい知見を得ることができ細胞接着の研究史に新しい事実を提供できました。また、細胞質分裂が正しく行われることは組織の恒常性の維持のために重要であり、分裂の失敗はゲノムの不安定性を引き起こして発がんの原因となります。多くのがん細胞は細胞間接着に異常をきたすことが知られており、フォルミン分子が接着部位への局在を失ったことによって分裂が失敗するという私たちの発見は発がんのメカニズムの一端を解明につながる可能性があります。
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