| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The centrosome functions not only as a microtubule formation center but also as a starting site for plasma membrane formation, but its molecular mechanism is still unclear.Since the formation of spore cell membranes in fission yeast begins from the centrosome (SPB in yeast), it was considered to be a model for cell membrane formation from the centrosome. The purpose of this study is to elucidate the molecular mechanism of the initiation of this membrane formation by combining genome-wide reverse genetics and biochemical methods. In this study, we revealed that calmodulin binds to Spo15 in a Ca2 + -dependent manner and localizes to SPB, thereby acting as a starting point for the spore cell membrane. Furthermore, we revealed the SPB protein to which calmodulin / Spo15 binds.
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