LINC complex functions in signal transduction pathways at the nuclear envelope
| Project/Area Number |
18K06226
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 44010:Cell biology-related
|
|---|
| Research Institution | Ehime Prefectural University of Health Science |
|---|
Principal Investigator |
Hieda Miki 愛媛県立医療技術大学, 保健科学部, 教授 (00380254)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
東山 繁樹 愛媛大学, プロテオサイエンスセンター, 教授 (60202272)
|
|---|
| Project Period (FY) |
2018-04-01 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 核膜 / LINC 複合体 / メカノトランスダクション / ゴルジ体 / 核小体 / 細胞核 / 核 / SUN / nesprin / lamin / 細胞接着装置 / 核膜タンパク質 / LINC複合体 / LINC complex / 情報伝達 / SUN1 |
|---|
| Outline of Final Research Achievements |
The primary functions of the nuclear envelope are to isolate the nucleoplasm and its contents from the cytoplasm; however, the nuclear envelope also plays a role in the transfer of various molecules and signals to and from the nucleus. Signal transduction across the nuclear envelope is essential for cellular functions such as transcriptional regulation and cell cycle progression, but remains poorly understood. LINC complex is a molecular bridge that spans the nuclear envelope and connects the nucleoskeleton and cytoskeleton. In this study, we revealed a regulation of the Golgi organization by the LINC complex. In addition, our study suggests that the LINC complex plays a role in modulating nucleolar morphology and numbers via chromatin. Moreover, we also revealed that LINC complex contributed to photon-enhanced cell migration and invasion. These results support a model that the LINC complex is capable of transmitting information bidirectionally between the nucleus and the cytoplasm.
|
| Academic Significance and Societal Importance of the Research Achievements |
細胞核は、遺伝情報の維持と複製、遺伝情報発現の出発点である転写を行うオルガネラである。本申請研究では、核膜に存在し、LINC複合体と呼ばれるタンパク質複合体の機能解明に取り組んだ。LINC複合体は、核内において核骨格と、細胞質において細胞骨格と結合し、細胞内における核の位置決定や、メカノトランスダクション、DNA修復、染色体動態の制御、細胞運動など多岐にわたる生理機能と、ある種の筋ジストロフィーや早老症、聴覚障害など組織を限定しない多様な疾病の発症に関与する。そのためLINC複合体が担う機能を明らかにすることはそれら発症機序の解明、治療法の開発へ繋がると期待される。
|
Report
(4 results)
Research Products
(21 results)
-
-
[Journal Article] The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal2021
Author(s)
Hieda, M. *, Matsumoto, T., Isobe, M., Kurono, S., Yuka, K., Kametaka, S., Wang, J.Y., Chi, Y.H., Kameda, K., Kimura, H., Matsuura, N., Matsuura, S.
-
Journal Title
Sci Rep.
Volume: 11
Issue: 1
Pages: 5358-5358
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
[Journal Article] X-ray-enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex2018
Author(s)
Imaizumi, H., Sato, S., Nishihara, A., Minami, K., Koizumi, M., Matsuura, N., Hieda, M
-
Journal Title
Cancer Science
Volume: 109
Issue: 4
Pages: 1158-1165
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
[Presentation] 子宮体癌におけるメチル化DNA結合タンパク質を用いた，DNAメチル化解析2019
Author(s)
坂本真吾, 末武勲, 窪田裕美, 門屋孝志, 三好陽子, 石原香菜子, 松本優衣, 髙石治彦, 水野洋輔, 大城由美, 檜枝美紀
Organizer
第58回日本臨床細胞学会秋期大会
Related Report
-
[Presentation] Functional analysis of RNA binding protein YB-1 involved in nuclear lobulation in HeLa cells2019
Author(s)
Kawabata, T., Ikeda, T., Noguchi, T., Hirata, H., Takamori, N., Komiya, I. Igarashi, M., Hieda, M., Tani, T.
Organizer
日本分子生物学会第42回年会
Related Report
-
-
-
-
-
-
[Presentation] LINC complex component, SUN1 play a role in the Golgi complex organization without nesprins2018
Author(s)
Matsumoto, T., Nishioka, Y., Isobe, M., Kametaka, S., Kimura, H., Matsuura, N., Hieda, M.
Organizer
第70回日本細胞生物学会（日本発生生物学会合同大会）
Related Report
-
