Functional Regulation of Migrating Sperm in the Mouse Oviduct
| Project/Area Number |
18K06241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44020:Developmental biology-related
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| Research Institution | University of Tsukuba |
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Principal Investigator |
BABA Tadashi 筑波大学, 生命環境系, 教授 (40165056)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 卵管 / 精子 / 受精能獲得 / マウス / 受精 |
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| Outline of Final Research Achievements |
To accomplish fertilization in the ampulla of oviduct, ejaculated sperm are required to migrate through the female reproductive tract. However, this fundamental process largely remains unknown. The purpose of this work was to elucidate the mechanism that regulates the function of mouse sperm during the migration in the oviduct. After natural mating, sperm were abundantly present in the utero-tubal junction. As sperm approached the ampulla, the number of sperm dramatically decreased; the sperm numbers in the isthmus and ampulla were found to be approximately 100 and 10-20, respectively. Thus, the transport of sperm from the utero-tubal junction to the isthmus may be mainly dependent on oviduct fluid flows generated by contractions, and the sperm migration may be switched by the sperm motility near the ampulla. Importantly, only a single acrosome-reacted sperm was located close to an unfertilized oocyte in the ampulla.
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| Academic Significance and Societal Importance of the Research Achievements |
顕微授精に代表される生殖補助技術の発展によって、雌性生殖管を経由しない精子でも産子が誕生することが証明された。このことを契機として、雌性生殖管での精子の機能制御に生理的意義がないような錯覚が広がっている。本研究では、生殖補助技術によってバイパスされた卵管で起こる仕組みを明らかにしていくところに高い学術的意義がある。加えて、ヒトや家畜・野生動物での生殖技術改善、避妊薬開発などの新しい技術開発への波及効果も期待できる。
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Calaxin is required for cilia-driven determination of vertebrate laterality2019
Author(s)
Sasaki Keita、Shiba Kogiku、Nakamura Akihiro、Kawano Natsuko、Satouh Yuhkoh、Yamaguchi Hiroshi、Morikawa Motohiro、Shibata Daisuke、Yanase Ryuji、Jokura Kei、Nomura Mami、Miyado Mami、Takada Shuji、Ueno Hironori、Nonaka Shigenori、Baba Tadashi、Ikawa Masahito、Kikkawa Masahide、Miyado Kenji、Inaba Kazuo
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Journal Title
Communications Biology
Volume: 2
Issue: 1
Pages: 226-226
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] SPAM1/HYAL5 Double Deficiency in Male Mice Leads to Severe Male Subfertility Caused by a Cumulus-Oocyte Complex Penetration Defect.2019
Author(s)
Park S, Kim YH, Jeong PS, Park C, Lee JW, Kim JS, Wee G, Song BS, Park BJ, Kim SH, Sim BW, Kim SU, Triggs-Raine B, Baba T, Lee SR, Kim E.
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Journal Title
FASEB J.
Volume: 33
Issue: 12
Pages: 14440-14449
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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