| Project/Area Number |
18K06249
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44020:Developmental biology-related
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NOBUHISA IKUO 東京医科歯科大学, 難治疾患研究所, 准教授 (40332879)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 造血幹細胞 / 転写因子 / 血液細胞塊 / Sox17 / 胎仔 / AGM / Gimap / hematopoietic stem cells / 造血 / NF-kappaB / Tet1 / 発生 |
|---|
| Outline of Final Research Achievements |
Hematopoietic stem cells, which are the source of all blood cells, first arise in hematopoietic cell clusters of the dorsal aorta in the embryonic day 10.5 of the mouse embryo. We analyzed genes and signaling pathways induced by Sox17, a transcription factor involved in the development, maturation and maintenance of hematopoietic stem cells in midgestation mouse embryos. We investigated the involvement of adherent molecules ESAM, the GTPase Gimap6, the signaling pathway activating NF-kappaB, and TET1 involved in DNA methylation, respectively.
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| Academic Significance and Societal Importance of the Research Achievements |
今回得られたマウス胎生中期における大動脈内腔の造血幹細胞を含む血液細胞塊の形成・成熟・維持にかかわる分子およびシグナル経路をさらに解析していくことにより、生体の造血幹細胞の特性と比較することにより、培養皿上での造血幹細胞の形成および維持機構の解明につながることが期待される。
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