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Analysis of ROS signaling in amphibian organ regeneration

Research Project

Project/Area Number 18K06257
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44020:Developmental biology-related
Research InstitutionNational Institute for Basic Biology (2020)
Hiroshima University (2018-2019)

Principal Investigator

Suzuki Ken-ichi  基礎生物学研究所, 新規モデル生物開発センター, 特任准教授 (90363043)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsイベリアトゲイモリ / 器官再生 / ROS / ゲノム編集 / トランスジェニック / 両生類 / トランスクリプトーム解析 / プロテオミクス / トランスクリプトミクス / エピジェネティクス
Outline of Final Research Achievements

The goal of this study is to elucidate the importance of ROS signaling in organ regeneration and the series of molecular mechanisms involved. We used the Iberian ribbed newt, which has a high capacity for organ regeneration and is capable of highly efficient genome editing, as a model animal. First, we performed transcriptomic analysis of the limb blastema to identify candidate factors downstream of ROS signaling that are key to limb regeneration. Next, we analyzed the knockout of some of these candidate genes and evaluated their functions in organ regeneration. In addition, we generated transgenic newt capable of spatiotemporally inducing ROS signaling at the cellular level.

Academic Significance and Societal Importance of the Research Achievements

器官再生におけるROSシグナルの重要性と分子機構を解明することが本申請課題の目的である。本研究課題では、高い器官再生能力を持ち、超高効率のゲノム編集が可能であるイベリアトゲイモリをモデルとして用いる。本研究から得られる成果は、発生・再生生物学におけるROSの新たな生物学的意義の発見につながる。くわえて、現在注目度の高い再生医学分野において、器官再生に関する重要な知見をもたらすことが期待できる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (3 results)

All 2020 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results)

  • [Journal Article] A simple and practical workflow for genotyping of CRISPR-Cas9‐based knockout phenotypes using multiplexed amplicon sequencing2020

    • Author(s)
      Midori Iida Miyuki Suzuki Yuto Sakane Hiroyo Nishide Ikuo Uchiyama Takashi Yamamoto Ken‐ichi T. Suzuki Satoshi Fujii
    • Journal Title

      Genes to Cells

      Volume: in press Issue: 7 Pages: 498-509

    • DOI

      10.1111/gtc.12775

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A comprehensive reference transcriptome resource for the Iberian ribbed newt Pleurodeles waltl, an emerging model for developmental and regeneration biology2019

    • Author(s)
      Matsunami Masatoshi、Suzuki Miyuki、Haramoto Yoshikazu、Fukui Akimasa、Inoue Takeshi、Yamaguchi Katsushi、Uchiyama Ikuo、Mori Kazuki、Tashiro Kosuke、Ito Yuzuru、Takeuchi Takashi、Suzuki Ken-ichi T、Agata Kiyokazu、Shigenobu Shuji、Hayashi Toshinori
    • Journal Title

      DNA Research

      Volume: 印刷中 Issue: 3 Pages: 217-229

    • DOI

      10.1093/dnares/dsz003

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cas9 ribonucleoprotein complex allows direct and rapid analysis of coding and noncoding regions of target genes in Pleurodeles waltl development and regeneration.2018

    • Author(s)
      Suzuki M., Hayashi T*., Inoue T., Agata K., Hirayama M., Suzuki M., Shigenobu S., Takeuchi T, Yamamoto T., and Suzuki KT*.
    • Journal Title

      Developmental Biology

      Volume: 447 Issue: 2 Pages: 127-136

    • DOI

      10.1016/j.ydbio.2018.09.008

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access

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Published: 2018-04-23   Modified: 2022-01-27  

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