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Regulatory mechanisms of germline stem cells in colonial ascidian

Research Project

Project/Area Number 18K06259
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44020:Developmental biology-related
Research InstitutionKochi University

Principal Investigator

Sunanaga Takeshi  高知大学, 教育研究部自然科学系理工学部門, 准教授 (20448393)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsSox / SSNA1 / 生殖系列幹細胞 / 配偶子形成 / トランスクリプトーム / 卵形成関連遺伝子 / sox / TDRD / Tudor / 卵母細胞 / 生殖細胞 / トランスクリプトーム解析 / 幹細胞 / ホヤ
Outline of Final Research Achievements

In this study, we analyzed the molecular mechanisms that control germline stem cell differentiation and germ cell formation by precise expression analysis and functional analysis of genes whose expression levels increase with the sexualization in the compound ascidian, Botryllus primigenus. It was clarified that the SSNA1 gene played an important role in the maintenance and/or differentiation control of germline stem cells. It was also shown that SSNA1, SoxB2, SoxF, and FoxL were expressed in oocytes and might be required for cell growth in oogenesis. Furthermore, Bre1a was identified as a gene expressed in germline stem cells, and eight genes showing germline-specific expression were identified. We obtained de novo transcriptomic data from this non-model organism and established informative database that contributes the progress of these genetic analyzes.

Academic Significance and Societal Importance of the Research Achievements

本研究でトランスクリプトームデータベースを構築したことは,ユニークな生活史をもつミダレキクイタボヤに対する今後の分子遺伝学的研究の発展に大きく貢献する。SSNA1が幹細胞の維持や分化制御に関わる例はこれまでに報告がない。よって,幹細胞生物学の基礎研究における新たな知見となる興味深いデータである。卵形成に関わる4つの遺伝子が明らかになった。特に,SSNA1およびSoxB2が生殖系列で発現している点は動物における初の報告であり,卵母細胞の成長に関わっている点も明らかになった。これらの成果は,生殖細胞形成機構を理解するうえでの重要な発見であると同時に,生殖医学に対する基盤的情報となり得るものである。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (7 results)

All 2020 2019 Other

All Int'l Joint Research (4 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] Villefranche-sur-mer Dev. Biol. Lab.(フランス)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Villefranche-sur-mer Dev. Biol. Lab.(フランス)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Villefranche-sur-mer Dev. Biol. Lab.(フランス)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Villefranche-sur-mer Dev. Biol. Lab.(フランス)

    • Related Report
      2018 Research-status Report
  • [Presentation] イタボヤ類の間充織細胞への遺伝子導入法の開発2020

    • Author(s)
      北橋翼 砂長 毅
    • Organizer
      日本分子生物学会 第43回日本分子生物学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] Role of Pumilio during Germ Cell Formation in Colonial Ascidian, Botryllus primigenus2019

    • Author(s)
      Takeshi Sunanaga
    • Organizer
      10th International Tunicate Meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] ミダレキクイタボヤにおける Sox遺伝子ファミリーの発現解析2019

    • Author(s)
      谷内 宥紀 砂長 毅
    • Organizer
      日本分子生物学会 第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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