| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
First of all we have generated live imaging system to analyze oocyte development. Using this system, we clarified that the cyst breakdown of oocytes was gradually progressing, and the cells going to be lost at this time were largely moved. Furthermore, we also generated mitochondria imaging ES cells (TREloxMtGR) which suggested that mitochondria are migrating between oocyte cysts.
On the other hand, we focused on the importance of primordial follicles which serve as storage for continuous ovulatory cycles. We found that primordial follicles are surrounded with extracellular matrix (ECM) which generated physical pressure. Whereas digestion of ECM induced oocyte activation, exogenous pressure prevented it.
From these findings we concluded that the pressure state is involved in the control of primordial follicles.
|
