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Study for wound epidermis signal to regulate regeneration

Research Project

Project/Area Number 18K06266
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44020:Developmental biology-related
Research InstitutionUniversity of Hyogo

Principal Investigator

Mochii Makoto  兵庫県立大学, 理学研究科, 准教授 (90202358)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsアフリカツメガエル / 再生 / 傷表皮 / apical epithelial cap / 分泌性シグナル因子 / AEC / 尾部再生 / トランスクリプトーム / 分泌生シグナル因子
Outline of Final Research Achievements

Wound epidermis (WE) and the apical epithelial cap (AEC) are believed to trigger regeneration of amputated appendages such as limb and tail in amphibians by producing certain secreted signaling molecules. Here we used an es1:egfp transgenic Xenopus laevis to isolate WE/AEC cells during the time course of tail regeneration. Transcriptome analysis revealed that more than 8,000 genes, including genes involved in several signaling pathways, displayed dynamic changes of their expression during tail regeneration. We identify seven secreted signaling molecule genes (mdk, fstl, slit1, tgfβ1, bmp7.1, angptl2 and egfl6) that are highly expressed in tail AEC cells. Among these genes, five were also highly expressed in limb AEC cells but the other two are specifically expressed in tail AEC cells. Interestingly, there was no expression of fgf8 in tail WE/AEC cells, whose expression and pivotal role in limb AEC cells have been reported previously.

Academic Significance and Societal Importance of the Research Achievements

本研究では、尾部の傷表皮/AECが比較的多種類の分泌因子を放出することおよび、尾部と肢芽とで分泌因子の組成が異なることを明らかにした。この発見は器官再生のメカニズム解明へ向けての重要なステップとなる 。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (4 results)

All 2021 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results) (of which Invited: 1 results)

  • [Journal Article] Cell type-specific transcriptome analysis unveils secreted signaling molecule genes expressed in apical epithelial cap during appendage regeneration.2019

    • Author(s)
      Okumura A, Hayashi T, Ebisawa M, Yoshimura M, Sasagawa Y, Nikaido I, Umesono Y, Mochii M.
    • Journal Title

      Dev. Growth Differ.

      Volume: 61 Issue: 9 Pages: 447-456

    • DOI

      10.1111/dgd.12635

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] 傷表皮で発現する分泌シグナル因子遺伝子の同定と機能解析2021

    • Author(s)
      餅井真
    • Organizer
      第三回 再生異分野融合研究会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] アフリカツメガエル幼生の尾部再生時に傷表皮で発現する分泌生シグナル因子遺伝子の解析2019

    • Author(s)
      菊本葵、奥村晃成、梅園良彦、餅井真
    • Organizer
      日本動物学会大90回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] アフリカツメガエル幼生尾部再生時の傷表皮/AECにおける神経の役割2018

    • Author(s)
      塚原由希菜、奥村晃成、梅園良彦、餅井真
    • Organizer
      日本動物学会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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