Transcriptional regulation of a gibberellin biosynthesis gene that regulates internode elongation in rice

• Project/Area Number: 18K06274
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44030:Plant molecular biology and physiology-related
• Research Institution: National Agriculture and Food Research Organization (2019-2020); Tohoku University (2018)
• Principal Investigator: Kuroha Takeshi 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 研究員 (50415155)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: イネ / ゲノム編集 / ジベレリン / 節間伸長 / 洪水応答 / エチレン / 洪水 / 生殖成長 / 環境応答 / 植物ホルモン / ストレス / イネ節間伸長 / 転写制御 / 深水応答

Outline of Final Research Achievements

Plants have diverse environmental response mechanisms in response to various external environments. Rice have the ability to elongate internodes in response to not only the onset of reproductive growth caused by such as day length and temperature, but also environmental changes caused by submergence. The gibberellin synthase gene SD1 (OsGA20ox2) has been identified as a common contributor to internode elongation. In this study, we generated genome-edited lines with various deletions in the untranslated region of the SD1 gene to elucidate the regulatory mechanism of the SD1 gene expression. Through phenotypic analysis of these lines, we succeeded in obtaining the candidate region involved in the regulation of internode elongation through the expression of the SD1 gene.

Academic Significance and Societal Importance of the Research Achievements

本研究成果により、イネの節間伸長の制御において重要なSD1遺伝子の発現制御機構を理解する上で大きな手がかりを得られたと言える。この成果は、植物の多様な成長戦略の解明に結びつくだけでなく、将来的にはイネをはじめとした作物における自在な草丈制御システムの構築に展開できる可能性がある。

Research Products

• [Int'l Joint Research] Cornell University(米国)
• [Journal Article] Molecular mechanisms and future improvement of submergence tolerance in rice (2020)
  • Author(s): Kuroha Takeshi、Ashikari Motoyuki
  • Journal Title: Molecular Breeding Volume: 40 Issue: 4 Pages: 41-41
  • DOI: 10.1007/s11032-020-01122-y
  • Peer Reviewed
• [Journal Article] 洪水に適応する浮イネの急速な節間伸長機構 (2020)
  • Author(s): 黒羽 剛, 芦苅基行
  • Journal Title: 植物の生長調節 Volume: 55 Pages: 32-39
  • NAID: 130008060508
• [Journal Article] Metabolite and Phytohormone Profiling Illustrates Metabolic Reprogramming as an Escape Strategy of Deepwater Rice during Partially Submerged Stress (2020)
  • Author(s): Fukushima Atsushi、Kuroha Takeshi、Nagai Keisuke、Hattori Yoko、Kobayashi Makoto、Nishizawa Tomoko、Kojima Mikiko、Utsumi Yoshinori、Oikawa Akira、Seki Motoaki、Sakakibara Hitoshi、Saito Kazuki、Ashikari Motoyuki、Kusano Miyako
  • Journal Title: Metabolites Volume: 10 Issue: 2 Pages: 68-68
  • DOI: 10.3390/metabo10020068
  • NAID: 120007132876
  • Peer Reviewed / Open Access
• [Journal Article] Ethylene-gibberellin signaling underlies adaptation of rice to periodic flooding (2018)
  • Author(s): T Kuroha, K Nagai, R Gamuyao, D R Wang, T Furuta, M Nakamori, T Kitaoka, K Adachi, A Minami, Y Mori, K Mashiguchi, Y Seto, S Yamaguchi, M Kojima, H Sakakibara, J Wu, K Ebana, N Mitsuda, M Ohme-Takagi, S Yanagisawa, M Yamasaki, R Yokoyama, K Nishitani, T Mochizuki, G Tamiya, S R McCouch, and M Ashikari
  • Journal Title: Science Volume: 361 Issue: 6398 Pages: 181-186
  • DOI: 10.1126/science.aat1577
  • NAID: 120006529624
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rice leaf hydrophobicity and gas films are conferred by a wax synthesis gene (LGF1) and contribute to flood tolerance. (2018)
  • Author(s): Kurokawa, Y., Nagai, K., Huan, P. D., Shimazaki, K., Qu, H., Mori, Y., Toda, Y., Kuroha, T., Hayashi, N., Aiga, S., Itoh, J.-i., Yoshimura, A., Sasaki-Sekimoto, Y., Ohta, H., Shimojima, M., Malik, A. I., Pedersen, O., Colmer, T. D. and Ashikari, M.
  • Journal Title: New Phytologist Volume: － Issue: 4 Pages: 1558-1569
  • DOI: 10.1111/nph.15070
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Time-Course Transcriptomics Analysis Reveals Key Responses of Submerged Deepwater Rice to Flooding (2018)
  • Author(s): Minami Anzu、Yano Kenji、Gamuyao Rico、Nagai Keisuke et. al.
  • Journal Title: Plant Physiology Volume: 176 Issue: 4 Pages: 3081-3102
  • DOI: 10.1104/pp.17.00858
  • Peer Reviewed / Int'l Joint Research
• [Presentation] イネの遺伝子機能の理解とその先に向けて (2019)
  • Author(s): 黒羽 剛
  • Organizer: 農学中手の会 第5回研究集会
• [Presentation] 浮きイネにおける深水に応答した代謝物変化の包括的解析 (2019)
  • Author(s): 黒羽 剛，草野都，福島敦史，永井啓祐，服部洋子，小林 誠，及川彰，小嶋美紀子，榊原 均，内海好規，関 原明，芦苅基行，斉藤和季
  • Organizer: 第 13 回メタボロームシンポジウム
• [Presentation] Molecular mechanism of adaptation to periodic flooding in rice. (2019)
  • Author(s): Takeshi Kuroha
  • Organizer: 35th ISPR International Symposium and 5th Symposium on Plant Stress Sciences
  • Int'l Joint Research / Invited
• [Presentation] An Ethylene-Gibberellin Relay Co-opts the Green Revolution Gene to Allow Rice Adaptation to Submergence. (2019)
  • Author(s): Takeshi Kuroha, Susan R. McCouch, and Motoyuki Ashikari
  • Organizer: International Plant & Animal Genome XXVII
  • Int'l Joint Research / Invited
• [Presentation] Ethylene-Gibberellin Signaling Underlies Adaptation of Rice to Periodic Flooding (2018)
  • Author(s): Takeshi Kuroha and Motoyuki Ashikari
  • Organizer: 16th International Symposium on Rice Functional Genomics
  • Int'l Joint Research
• [Presentation] 長期的な洪水に適応した浮きイネの起源とその栽培化 (2018)
  • Author(s): 黒羽 剛, Diane Wang, Susan R. McCouch, 横山隆亮, 西谷和彦, 芦苅基行
  • Organizer: 日本育種学会第134回講演会
• [Presentation] 浮イネにおけるジベレリン合成を介した洪水への適応戦略 (2018)
  • Author(s): 黒羽 剛，増口 潔，瀬戸義哉，山口信次郎，横山隆亮，西谷和彦，芦苅基行
  • Organizer: 日本植物学会第82回大会