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Study on mechanisms to maintain the function of photosystem I under strong light

Research Project

Project/Area Number 18K06275
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionSaitama University

Principal Investigator

Takahashi Hiroko  埼玉大学, 理工学研究科, 助教 (50748126)

Co-Investigator(Kenkyū-buntansha) 西山 佳孝  埼玉大学, 理工学研究科, 教授 (30281588)
Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords光化学系I / チラコイド膜タンパク質 / 電子伝達 / クラミドモナス / 光阻害 / PGRL1タンパク質 / 光合成 / 光化学系 / Photosystme I
Outline of Final Research Achievements

A thylakoid membrane protein, PGRL1 contains six cysteine residues conserved from green algae to land plants. In PGRL1-deficient mutant (pgrl1), photosystem I (PSI) is sensitive to inactivation under the strong light. To reveal the mechanism by which PGRL1 protects PSI against strong light in Chlamydomonas, we generated the complemented strains and the strains substituted from cysteine to serine residues in PGRL1 (CS variants). Under strong light, the activity of PSI in C63SC1673 variant was remained high, but the activity in C256SC269S and C284SC287S variants declined, as observed in pgrl1. Based on our results, the substitutions affected the PSI photoprotection more severely than lowering the level of PGRL1, though some CS variants showed lower accumulation of PGRL1. Thus, the cysteine residues in the C-terminal region of PGRL1 seems to play important roles in protecting PSI under strong light.

Academic Significance and Societal Importance of the Research Achievements

光は光合成に必要であるが、強い光は細胞内での活性酸素種の発生を引き起こすなど有害なストレスとなる。二つの光化学系のうち(PSI, PSII)、PSIIは強光ストレスに弱く容易に失活する一方、PSIは強光下でも活性を維持することが知られていたため、PSI光阻害については研究報告はかなり限られていた。本研究では、PGRL1タンパク質及びそのC末端部位に保存されるシステイン残基がPSIの活性維持に果たす役割を明らかになり、PSI活性維持メカニズムの解明の一旦を担った。光阻害の予防は農学的にも重要であるため、本研究成果は育種等の応用に向けた分子基盤を提供できる可能性がある。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2023 2021 2020 2019

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (7 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Journal Article] The NAD Kinase Slr0400 Functions as a Growth Repressor in Synechocystis sp. PCC 68032021

    • Author(s)
      Ishikawa Yuuma、Cassan Cedric、Kadeer Aikeranmu、Yuasa Koki、Sato Nozomu、Sonoike Kintake、Kaneko Yasuko、Miyagi Atsuko、Takahashi Hiroko、Ishikawa Toshiki、Yamaguchi Masatoshi、Nishiyama Yoshitaka、Hihara Yukako、Gibon Yves、Kawai-Yamada Maki
    • Journal Title

      Plant and Cell Physiology

      Volume: - Issue: 4 Pages: 1-10

    • DOI

      10.1093/pcp/pcab023

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection2021

    • Author(s)
      Lu Yandu、Gan Qinhua、Iwai Masakazu、Alboresi Alessandro、Burlacot Adrien、Dautermann Oliver、Takahashi Hiroko、Crisanto Thien、Peltier Gilles、Morosinotto Tomas、Melis Anastasios、Niyogi Krishna K.
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 679-688

    • DOI

      10.1038/s41467-021-20967-1

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] クラミドモナスPGRL1がPSI光防御に果たす役割2023

    • Author(s)
      高橋拓子、髙山健太、井須敦子、若林憲一、久堀徹、西山佳孝
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] クラミドモナスPGRL1の機能解析を目指して2021

    • Author(s)
      髙橋拓子
    • Organizer
      第11回日本光合成学会年会シンポジウム
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] PGRL1 involves in the stable PSI activity under high light in the green alga Chlamydomonas reinhardtii2021

    • Author(s)
      Hiroko Takahashi
    • Organizer
      Finland-Japan binational seminar
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] クラミドモナスPGRL1システイン残基の機能解析2021

    • Author(s)
      高橋拓子、高山健太、井須敦子、若林憲一、久堀徹、西山佳孝
    • Organizer
      第62回日本植物生理学会年会 一般講演
    • Related Report
      2020 Research-status Report
  • [Presentation] Cyclic electron flow A to Z2020

    • Author(s)
      Hiroko Takahashi
    • Organizer
      第84回日本植物学会年会 JPRシンポジウム
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 緑藻クラミドモナスにおけるPGRL1の生化学的解析2020

    • Author(s)
      高橋拓子、高山健太、井須敦子、若林憲一、久堀徹、西山佳孝
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Role of PGRL1 in photoprotection of PSI in the green alga Chlamydomonas reinhardtii2019

    • Author(s)
      Hiroko Takahashi, Kenta Takayama, Atsuko Isu, Ken-ichi Wakabayashi, Toru Hisabori and Yoshitaka Nishiyama
    • Organizer
      1st Japan-US Binational Seminar (Presented by Grant-in-Aid for Scientific Research on Innovative Areas "New Photosynthesis"
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2024-01-30  

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