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Roles of polysaccharides in the development of pollen exine 3D structure

Research Project

Project/Area Number 18K06281
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionNagoya University

Principal Investigator

Ishiguro Sumie  名古屋大学, 生命農学研究科, 准教授 (50260039)

Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords花粉 / 細胞壁 / 立体構造形成 / 多糖 / 突然変異体 / シロイヌナズナ / エキシン / スポロポレニン / キシラン / COPII小胞 / 小胞体 / タペート細胞 / ペクチン / アラビノガラクタンタンパク質 / 細胞内小胞輸送 / Ypt/Rab GTPase / 植物発生学 / 花粉形態形成 / 細胞壁多糖 / 生殖 / 雄性不稔
Outline of Final Research Achievements

Pollen grains of flowering plants are covered with elaborate 3D structures called exine, of which light and robust features are suitable for protecting male gametes. The exine structure is species specific, indicating that the structure is determined by genes. In this study, we focused on the mechanism how Arabidopsis makes their reticulate exine, especially on the function of polysaccharides. We found that xylan and pectin made a module and localized in the mesh of developing exine, whereas arabinogalactan proteins wrapped the exine net. The mutants deficient in these polysaccharides made collapsed exine. We concluded that the polysaccharides work as a frame of developing exine.

Academic Significance and Societal Importance of the Research Achievements

本研究の意義は、生物が遺伝情報に基づいて種特異的な形を作るしくみの一端を解明したことである。生物の形作りにおいても、エキシンのように細胞の外に特定の構造を作るという現象は珍しい。細胞外に分泌された多糖が脂質性高分子化合物を成形する鋳型として働くという、これまで知られていなかったメカニズムが明らかになった。背景にあるのは分子の自己集合と自己組織化であり、直ちに応用展開できる成果ではないものの、自律的な組織形成に基づいた有用構造物の生産に資する基盤的な知見である。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (12 results)

All 2023 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 2 results) Remarks (2 results)

  • [Int'l Joint Research] 上海交通大学(中国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] 上海交通大学(中国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] La Trobe University(オーストラリア)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Targeted expression of bgl23-D, a dominant-negative allele of ATCSLD5, affects cytokinesis of guard mother cells and exine formation of pollen in Arabidopsis thaliana2023

    • Author(s)
      Hossain Md. Firose、Dutta Amit Kumar、Suzuki Takamasa、Higashiyama Tetsuya、Miyamoto Chiharu、Ishiguro Sumie、Maruta Takanori、Muto Yuki、Nishimura Kohji、Ishida Hideki、Aboulela Mostafa、Hachiya Takushi、Nakagawa Tsuyoshi
    • Journal Title

      Planta

      Volume: 257 Issue: 4 Pages: 64-82

    • DOI

      10.1007/s00425-023-04097-0

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] NERD1 is required for primexine formation and plasma membrane undulation during microsporogenesis in Arabidopsis thaliana2020

    • Author(s)
      Xu Dawei、Mondol Palash Chandra、Ishiguro Sumie、Shi Jianxin、Zhang Dabing、Liang Wanqi
    • Journal Title

      aBIOTECH

      Volume: 1 Issue: 4 Pages: 205-218

    • DOI

      10.1007/s42994-020-00022-1

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] シロイヌナズナのCOPII被覆小胞形成因子KNS21の花粉エキシン形成における役割2023

    • Author(s)
      鍋田紗妃, 上木望路, 松岡耕汰, 中川強, 石黒澄衞
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Contribution of xylan and arabinogalactan proteins to pollen exine development2021

    • Author(s)
      Sumie Ishiguro
    • Organizer
      The 7th International Conference on Plant Cell Wall Biology
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] タペート細胞に発言するBOR1は花粉形成に重要である2019

    • Author(s)
      松本真季, 山崎有紗. 吉成晃, 石黒澄衞, 高野順平
    • Organizer
      日本土壌肥料学会2019年度静岡大会
    • Related Report
      2019 Research-status Report
  • [Presentation] スイレン由来アルカン合成酵素によるタバコ培養細胞BY-2でのアルカンの生産2019

    • Author(s)
      小島久恵, 鈴木孝征, 石黒澄衞
    • Organizer
      第32回植物脂質シンポジウム
    • Related Report
      2019 Research-status Report
  • [Presentation] Contribution of cell wall polysaccharides to the construction of the reticulate exine structure of pollen grains in Arabidopsis2018

    • Author(s)
      Sumie Ishiguro, Masayuki Yasutomi, Toshiya Suzuki, Joan Onate Narciso, Kyoko Esaki, Wei Zeng, Monika S Doblin, Antony Bacic
    • Organizer
      The 25th International Congress on Sexual Plant Reproduction
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Remarks] 名古屋大学大学院生命農学研究科 生物化学研究室ウェブサイト

    • URL

      https://www.agr.nagoya-u.ac.jp/~biochem/

    • Related Report
      2019 Research-status Report
  • [Remarks] 研究室ウェブサイト

    • URL

      https://www.agr.nagoya-u.ac.jp/~biochem/ishiguro.html

    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2024-01-30  

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