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Analysis of membrane trafficking in sucrose-dependent seedling establishment

Research Project

Project/Area Number 18K06284
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionKyoto University

Principal Investigator

Shimada Tomoo  京都大学, 理学研究科, 講師 (20281587)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords膜交通 / 液胞輸送 / シロイヌナズナ / kam2変異体 / ショ糖 / 変異体
Outline of Final Research Achievements

When Arabidopsis kam2 mutants are germinated on a sucrose-free solid medium, a certain percentage of individuals fail to develop true leaves after cotyledon expansion (this phenotype is called sucrose-requiring). This study revealed that the kam2 mutant phenotype was more prone to accumulation of excess water in the body and growth arrest (so-called vitrification) than the wild type, indicating an apparent sucrose requirement. The addition of sucrose to the medium may act to compensate for the defects caused by abnormal transport in kam2. Apparent sucrose requirement was also observed in wild-type Arabidopsis, suggesting that it may be an environmental response in which plants germinated in an unfavorable environment temporarily stop growing.

Academic Significance and Societal Importance of the Research Achievements

植物細胞における膜交通の分子基盤は、動物細胞や酵母など他の真核生物のそれとは大きくは変わらない。一方で、植物は固有の発生や生理、環境応答に対応して独自の膜交通システムを発展させていることが知られている。本研究では、kam2変異体をショ糖を含まない培地で育てることにより、輸送異常による細胞内の恒常性の破綻が観察できることが明らかとなった。発芽成長という植物固有の発生や生理に対応して、植物が独自に発展させてきた膜交通の役割の一端を明らかにすることができた。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (4 results)

All 2021 2020

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results)

  • [Journal Article] Spatiotemporal relationship between auxin dynamics and hydathode development in Arabidopsis leaf teeth2021

    • Author(s)
      Yagi Hiroki、Tamura Kentaro、Matsushita Tomonao、Shimada Tomoo
    • Journal Title

      Plant Signaling and Behavior

      Volume: 16 Issue: 12 Pages: 1989216-1989216

    • DOI

      10.1080/15592324.2021.1989216

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fluorescent protein-based imaging and tissue-specific RNA-seq analysis of Arabidopsis hydathodes2020

    • Author(s)
      Yagi Hiroki、Nagano Atsushi J、Kim Jaewook、Tamura Kentaro、Mochizuki Nobuyoshi、Nagatani Akira、Matsushita Tomonao、Shimada Tomoo
    • Journal Title

      Journal of Experimental Botany

      Volume: 72 Issue: 4 Pages: 1260-1270

    • DOI

      10.1093/jxb/eraa519

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 液胞輸送変異体kam2における 発芽後の成長阻害の解析2021

    • Author(s)
      細川智佳,田村謙太郎,岡義人,松下智直,嶋田知生
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] 液胞輸送変異体 kam2の芽生えにおけるショ糖要求性の表現型解析2020

    • Author(s)
      細川智佳,田村謙太郎,嶋田知生
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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