Transcriptional and post-translational regulation of germ cell differentiation in land plants

• Project/Area Number: 18K06285
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44030:Plant molecular biology and physiology-related
• Research Institution: Kyoto University
• Principal Investigator: Yamaoka Shohei 京都大学, 生命科学研究科, 准教授 (00378770)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 生殖細胞分化 / 配偶子形成 / 転写因子 / 環境応答 / 陸上植物 / ゼニゴケ / 花粉 / 生殖細胞 / 発生・分化

Outline of Final Research Achievements

The BONOBO (BNB) family of transcription factor are evolutionarily conserved regulators of plant germline cell differentiation. In this study, transcriptional and post-translational regulation of BNB gene expression were analyzed. In the liverwort Marchantia polymorpha, I showed that MpBNB transcription is regulated by the orthologs of key regulators for flowering in response to day length and light quality. It was also shown that Arabidopsis BNB2 is transcriptionally activated in an early stage of flower bud development. Both of Marchantia and Arabidopsis BNB proteins form a heterodimer with the other transcription factors to regulate germline cell differentiation. Further analyses suggested that this heterodimer is relatively stable in an early stage of M. polymorpha gametangium development, while it is transiently formed during fate determination of generative cell in Arabidopsis pollen.

Academic Significance and Societal Importance of the Research Achievements

植物の配偶子形成機構は、未同定の基本的制御因子がいまだ多いため、不明な部分が多い。特にコケ植物などの基部植物の配偶子形成とその光環境依存的制御の分子機構はほとんど明らかでない。本研究は、最近研究代表者らが同定した鍵因子BNBの機能発現調節機構についての基礎的知見を得ることで、こうした問題の解明を目指すものである。こうした知見は、食糧生産の基礎である植物有性生殖のメカニズムの全容解明に不可欠であり、社会的意義は大きい。

Research Products

• [Int'l Joint Research] Gregor Mendel Institute(オーストリア)
• [Int'l Joint Research] University of Kentucky(米国)
• [Journal Article] Development and Molecular Genetics of Marchantia polymorpha. (2021)
  • Author(s): Kohchi T, Yamato KY, Ishizaki K, Yamaoka S, Nishihama R.
  • Journal Title: Annu. Rev. Plant Biol. Volume: 72 Issue: 1 Pages: 677-702
  • DOI: 10.1146/annurev-arplant-082520-094256
  • Peer Reviewed
• [Journal Article] Chromatin Organization in Early Land Plants Reveals an Ancestral Association between H3K27me3, Transposons, and Constitutive Heterochromatin (2020)
  • Author(s): Montgomery SA, Tanizawa Y, Galik B, Wang N, Ito T, Mochizuki T, Akimcheva S, Bowman JL, Cognat V, Drouard L, Ekker H, Houng SF, Kohchi T, Lin SS, Liu LYD, Nakamura Y, Valeeva LR, Shakirov EV, Shippen DE, Wei WL, Yagura M, Yamaoka S, Yamato KT, Liu C, Berger F.
  • Journal Title: Current Biology Volume: 30 Issue: 4 Pages: 573-588
  • DOI: 10.1016/j.cub.2019.12.015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular mechanism and evolution of gametogenesis in land plants (2019)
  • Author(s): 山岡 尚平、河内 孝之、荒木 崇
  • Journal Title: 生化学 Volume: 91 Issue: 4 Pages: 534-539
  • DOI: 10.14952/SEIKAGAKU.2019.910534
  • NAID: 40022022830
  • ISSN: 0037-1017
  • Year and Date: 2019-08-25
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of the Poly(A) Status of Mitochondrial mRNA by Poly(A)-Specific Ribonuclease Is Conserved among Land Plants (2019)
  • Author(s): Kanazawa Mai、Ikeda Yoko、Nishihama Ryuichi、Yamaoka Shohei、Lee Nam-Hee、Yamato Katsuyuki T、Kohchi Takayuki、Hirayama Takashi
  • Journal Title: Plant and Cell Physiology Volume: 61 Issue: 3 Pages: 470-480
  • DOI: 10.1093/pcp/pcz212
  • NAID: 120006900804
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] An Early Arising Role of the MicroRNA156/529-SPL Module in Reproductive Development Revealed by the Liverwort Marchantia polymorpha (2019)
  • Author(s): Tsuzuki Masayuki、Futagami Kazutaka、Shimamura Masaki、Inoue Chikako、Kunimoto Kan、Oogami Takashi、Tomita Yuki、Inoue Keisuke、Kohchi Takayuki、Yamaoka Shohei、Araki Takashi、Hamada Takahiro、Watanabe Yuichiro
  • Journal Title: Current Biology Volume: 29 Issue: 19 Pages: 3307-3314
  • DOI: 10.1016/j.cub.2019.07.084
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Building new insights in plant gametogenesis from an evolutionary perspective (2019)
  • Author(s): Hisanaga Tetsuya、Yamaoka Shohei、Kawashima Tomokazu、Higo Asuka、Nakajima Keiji、Araki Takashi、Kohchi Takayuki、Berger Fr?d?ric
  • Journal Title: Nature Plants Volume: 5 Issue: 7 Pages: 663-669
  • DOI: 10.1038/s41477-019-0466-0
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A cis-acting bidirectional transcription switch controls sexual dimorphism in the liverwort. (2019)
  • Author(s): Hisanaga T, Okahashi K, Yamaoka S, Kajiwara T, Nishihama R, Shimamura M, Yamato KT, Bowman JL, Kohchi T, Nakajima K.
  • Journal Title: EMBO J. Volume: 38 Issue: 6
  • DOI: 10.15252/embj.2018100240
  • NAID: 120006547401
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Loss of CG Methylation in Marchantia polymorpha Causes Disorganization of Cell Division and Reveals Unique DNA Methylation Regulatory Mechanisms of Non-CG Methylation. (2018)
  • Author(s): Ikeda Y, Nishihama R, Yamaoka S, Arteaga-Vazquez MA, Aguilar-Cruz A, Grimanelli D, Pogorelcnik R, Martienssen RA, Yamato KT, Kohchi T, Hirayama T, Mathieu O.
  • Journal Title: Plant Cell Physiol. Volume: 12 Pages: 2421-2431
  • DOI: 10.1093/pcp/pcy161
  • Peer Reviewed / Int'l Joint Research
• [Presentation] ゼニゴケから花粉の雄性配偶子形成のメカニズムを知る (2021)
  • Author(s): 山岡 尚平
  • Organizer: 第62回日本植物生理学会年会・関連集会「植物生殖改変ワークショップ」
• [Presentation] 苔類ゼニゴケの遠赤色光・日長依存的な成長相転換の分子機構の解析 (2021)
  • Author(s): 金坂 侑紀，井上 佳祐，山岡 尚平，荒木 崇
  • Organizer: 第62回日本植物生理学会年会
• [Presentation] Analysis of GH17 members regulating engulfment of the generative cell into the vegetative cell in Arabidopsis (2020)
  • Author(s): Ebine K, Yamaoka S, Ueda T.
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] Deep evolutionary origin of gamete-directed zygote activation by KNOX/BELL transcription factors in green plants (2020)
  • Author(s): Hisanaga T, Fujimoto S, Cui Y, Sato K, Yamaoka S, Kohchi K, Berger F, Nakajima K.
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] Analysis of germ cell-specific histone H1 variants involved in spermiogenesis in Marchantia (2020)
  • Author(s): Kotani K, Nishida R, Higo A, Yamaoka S, Inoue K, Araki T.
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] Chromosome-level genome assembly of liverwort Marchantia polymorpha and updates of the genome database MarpolBase (2020)
  • Author(s): Tanizawa Y, Yagura M, Sakamoto M, Mochizuki T, Montgomery SA, Galik B, Yamaoka S, Nishihama R, Yamato KT, Kohchi T, Berger F, Liu C, Nakamura Y.
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] Evolutionary Scenario behind Bacterial-to-Eukaryotic Takeover of Chloroplast DNA Ligase Function in Land Plants (2020)
  • Author(s): Hamaji T, Kobayashi Y, Yamaoka S, Shikanai T, Nishimura Y.
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] フィトクロムを介した遠赤色光シグナルはゼニゴケの生殖器官形成を制御する (2019)
  • Author(s): 井上 佳祐、金坂 侑紀、山岡 尚平、西浜 竜一、河内 孝之、荒木 崇
  • Organizer: 日本植物学会第83回大会
• [Presentation] ゼニゴケの生殖器官形成における光質と日長の役割 (2019)
  • Author(s): 金坂 侑紀，井上 佳祐，山岡 尚平，西浜 竜一，河内 孝之，荒木 崇
  • Organizer: 日本植物学会第83回大会
• [Presentation] 緑色植物における葉緑体DNAリガーゼのバクテリア型から真核生物型への機能移譲 (2019)
  • Author(s): 浜地 貴志，小林 優介，山岡 尚平，鹿内 利治，西村 芳樹
  • Organizer: 日本植物学会第83回大会
• [Presentation] アンチセンスlong non-coding RNA SUFによる苔類ゼニゴケ雌性化因子MpFGMYB の発現抑制メカニズムの解明 (2019)
  • Author(s): 梶原 智明，岡橋 啓太郎，久永 哲也，岩_ 美雪，宮崎 基，山岡 尚平，西浜 竜一，嶋村 正樹，大和 勝幸，中島 敬二，河内 孝之
  • Organizer: 日本植物学会第83回大会
• [Presentation] A histidine kinase MpCKI1 plays a role in female sexual development in Marchantia polymorpha (2019)
  • Author(s): 包 昊南，吉竹 良洋，山岡 尚平，西浜 竜一，河内 孝之
  • Organizer: 日本植物学会第83回大会
• [Presentation] SABATH ファミリー遺伝子を介した生殖器官形成の抑制 (2019)
  • Author(s): 川村 昇吾，山岡 尚平，Rui Sun，楠 龍之介，西浜 竜一，河内 孝之
  • Organizer: 日本植物学会第83回大会
• [Presentation] Evolutionarily Conserved Transcription Factors Involved in Gametangium Development in Marchantia polymorpha (2019)
  • Author(s): Yamaoka S, Inoue K, Saito M, Yoshitake Y, Nishihama R, Kohchi T, Araki T.
  • Organizer: Marchantia Workshop 2019
  • Int'l Joint Research / Invited
• [Presentation] Bacterial-to-Eukaryotic Takeover of Chloroplast DNA Ligase Function in Land Plants (2019)
  • Author(s): Hamaji T, Kobayashi Y, Yamaoka S, Shikanai T, Nishimura Y.
  • Organizer: Marchantia Workshop 2019
  • Int'l Joint Research / Invited
• [Presentation] Analysis of the Mechanism How Antisense lncRNA SUF Represses the Female Promoting Factor MpFGMYB in Marchantia polymorpha (2019)
  • Author(s): Kajiwara T, Okahashi K, Hisanaga T, Miyazaki M, Iwasaki M, Yamaoka S, Nishihama R, Shimamura M, Yamato KT, Nakajima K, Kohchi T.
  • Organizer: Marchantia Workshop 2019
  • Int'l Joint Research
• [Presentation] Regulation of Sexual Organ Development by Methyltransferase of Gibberellin-Related Compounds in Marchantia polymorpha (2019)
  • Author(s): Kawamura S, Yamaoka S, Sun R, Yoshitake Y, Nishihama R, Kohchi T.
  • Organizer: Marchantia Workshop 2019
  • Int'l Joint Research
• [Presentation] Update of MarpolBase to accommodate ver. 5.1 gene annotation (2019)
  • Author(s): Tanizawa Y, Yagura M, Sakamoto M, Mochizuki T, Montgomery SA, Galik B, Yamaoka S, Yamato KT, Kohchi T, Nakamura Y, Liu C, Berger F.
  • Organizer: Marchantia Workshop 2019
  • Int'l Joint Research
• [Presentation] Tissue Specificity of Far-Red Light Signaling Pathway in Marchantia polymorpha (2019)
  • Author(s): Morisaki R, Yoshitake Y, Inoue K, Yamaoka S, Nishihama R, Kohchi T.
  • Organizer: Marchantia Workshop 2019
  • Int'l Joint Research
• [Presentation] The DELLA Protein Regulates Growth and Far-Red Light Response in the Liverwort Marchantia polymorpha (2019)
  • Author(s): Sun R, Hern_ndez-Garc_a J, Inoue K, Yamaoka S, NishihamaR, Bl_zquez MA, Kohchi T.
  • Organizer: Marchantia Workshop 2019
  • Int'l Joint Research
• [Presentation] Exploring the Function of the miR390-TAS3-ARF Pathway in Marchantia polymorpha (2019)
  • Author(s): Hung YL, Chien YC, Hainiwa E, Sun R, Yamaoka S, Nishihama R, Kohchi T, Wang CN.
  • Organizer: Marchantia Workshop 2019
  • Int'l Joint Research
• [Presentation] Gibberellin Precursor Is Involved in Sexual Organ Formation in the Liverwort Marchantia polymorpha (2019)
  • Author(s): Rui Sun, Ran Wang, Ryunosuke Kusunoki, Keisuke Inoue, Ryuichi Nishihama, Shohei Yamaoka, Takayuki Kohchi
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] メチル基転移酵素ファミリー遺伝子による苔類ゼニゴケの生殖器官形成の制御 (2019)
  • Author(s): 川村昇吾，山岡尚平，楠龍之介，Rui Sun，西浜竜一，河内孝之
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] 苔類ゼニゴケにおけるX染色体上の性決定遺伝子の探索 (2019)
  • Author(s): 岩崎美雪，山岡尚平，梶原智明，宮﨑基，末次憲之，吉竹良洋，西浜竜一，大和勝幸，河内孝之
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] MpBONOBO 転写複合体による苔類ゼニゴケの生殖器官形成の制御 (2019)
  • Author(s): 齊藤美咲，山岡尚平，吉竹良洋，光田展隆，西浜竜一，河内孝之
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] 異所的な分裂組織を形成するゼニゴケeda1変異体の解析 (2019)
  • Author(s): 毛利遊野，大谷健人，山岡尚平，西浜竜一，河内孝之，高橋卓，本瀬宏康
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] ゼニゴケMarchantiapolymorphaと珪藻Nitzschiasp.NIES4239のゲノム解析 (2019)
  • Author(s): 望月孝子，谷澤靖洋，山岡尚平，西浜竜一，金澤建彦，Sean A. Montgomery，Chang Liu， Bence Galik，Frederic Berger，上田貴志，大和勝幸，河内孝之，谷藤吾朗，神川龍馬，中村保一
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] 陸上植物の生殖細胞分化に必要な転写因子BONOBOの同定と標的遺伝子の探索 (2018)
  • Author(s): 山岡尚平, 西浜竜一, 吉竹良洋, 石田咲子, 井上佳祐, 齋藤美咲, 岡橋啓太郎, 包昊南, 西田浩之, 山口勝司, 重信秀治, 石崎公庸, 大和勝幸, 河内孝之
  • Organizer: 日本植物学会第82回大会
• [Presentation] ゼニゴケにおけるDNAメチル化制御 (2018)
  • Author(s): 池田陽子, 西浜竜一, 山岡尚平, ARTEAGA‐VAZQUEZ Mario. A, GRIMANELLI Daniel, MARTIENSSEN Robert A., POGORELCNIK Romain, MATHIEU Olivier, 大和勝幸, 河内孝之, 平山隆志
  • Organizer: 日本植物学会第82回大会
• [Presentation] 母性遺伝メカニズムの解明に向けたクラミドモナス有性生殖のゲノム編集アプローチ (2018)
  • Author(s): 浜地貴志, 山岡尚平, 鹿内利治, 西村芳樹
  • Organizer: 日本植物学会第82回大会
• [Presentation] 転写因子BONOBOの機能に必要な相互作用因子の同定 (2018)
  • Author(s): 齊藤美咲, 山岡尚平, 吉竹良洋, 光田展隆, 西浜竜一, 河内孝之
  • Organizer: 日本植物学会第82回大会
• [Presentation] クローナル解析で究明する苔類ゼニゴケの無性芽発生パターンとオーキシン受容体MpTIR1の役割 (2018)
  • Author(s): 鈴木秀政, HARRISON Jill, 嶋村正樹, 山岡尚平, 河内孝之, 西浜竜一
  • Organizer: 日本植物学会第82回大会
• [Remarks] 「陸上植物における配偶子形成とその進化」に関する総説を発表
  • URL: http://www.lif.kyoto-u.ac.jp/j/?post_type=research&p=12138