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Analysis of new transcriptional gene silencing mechanism in plants

Research Project

Project/Area Number 18K06291
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionOkayama University

Principal Investigator

Ikeda Yoko  岡山大学, 資源植物科学研究所, 准教授 (80467688)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsサイレンシング / 植物 / エピジェネティクス / 転写サイレンシング / エピゲノム / 遺伝子サイレンシング / トランスポゾン / シロイヌナズナ
Outline of Final Research Achievements

Plant mobile domain(PMD)protein, MAIL1 and MAIN, are required for TGS independent of DNA methylation and siRNAs. To understand the precise mechanism of TGS regulation by MAIL1 and MAIN, we did suppressor screening of mail1 mutant to isolate factors that are related to TGS regulation by MAIL1 and MAIN. To reveal the subnuclear functions of PMD proteins, we also tried to analyze 3D chromatin structure by chromatin conformation capture technology and open chromatin region.

Academic Significance and Societal Importance of the Research Achievements

ウイルスやトランスポゾンといった侵略者と、宿主ゲノムの間では、活性化と抑制のせめぎ合いが絶えず行われて来たと考えられ、この現象は生物のゲノム進化の過程で重要な役割を果たした可能性がある。本研究はこの機構が植物進化の過程に関与してきたことを示唆するゲノム進化のモデルケースとして興味深い。また、本研究で明らかになった新規サイレンシング機構は植物の遺伝子発現調節の新たな系として利用できる可能性がある。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (13 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (4 results) Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (7 results) (of which Invited: 3 results)

  • [Int'l Joint Research] Universite Clermont Auvergne(フランス)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Universite Clermont Auvergne(フランス)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Universite Clermont Auvergne(フランス)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Universite Clermont Auvergne(フランス)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Reinvention of hermaphroditism via activation of a RADIALIS-like gene in hexaploid persimmon2022

    • Author(s)
      Masuda Kanae、Ikeda Yoko、Matsuura Takakazu、Kawakatsu Taiji、Tao Ryutaro、Kubo Yasutaka、Ushijima Koichiro、Henry Isabelle M.、Akagi Takashi
    • Journal Title

      Nature Plants

      Volume: 8 Issue: 3 Pages: 217-224

    • DOI

      10.1038/s41477-022-01107-z

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Single-cell RNA-seq analysis reveals ploidy-dependent and cell-specific transcriptome changes in Arabidopsis female gametophytes2020

    • Author(s)
      Song Qingxin、Ando Atsumi、Jiang Ning、Ikeda Yoko、Chen Z. Jeffrey
    • Journal Title

      Genome Biology

      Volume: 21 Issue: 1 Pages: 178-178

    • DOI

      10.1186/s13059-020-02094-0

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 圃場オオムギにおけるヒストン修飾と遺伝子発現のゲノムワイド時系列解析2021

    • Author(s)
      (2)池田陽子・岡田聡史・金谷麻加・井上小槙・最相大輔・井藤純・辻寛之・持田恵一・平山隆志
    • Organizer
      第62回日本植物生理学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] シロイヌナズナにおける脱メチル化酵素を用いたDNAメチル化編集技術の開発2021

    • Author(s)
      (10)平田峻也・大河優奈・若林荘太朗・高橋広夫・町田千代子・池田陽子・西村泰介・小林括平・賀屋秀隆
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Analysis for Gene Silencing Mechanism by Plant Mobile Domain Proteins in Arabidopsis.2020

    • Author(s)
      Ikeda, Y. and Mathieu, O.
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] 植物のエピゲノム制御メカニズムとその利用に向けて.2020

    • Author(s)
      池田陽子
    • Organizer
      令和2年度植物科学4拠点アライアンス交流会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] ゼニゴケにおけるDNAメチル化制御2019

    • Author(s)
      池田陽子・西浜 竜一・山岡尚平・Mario A. Arteaga-Vazquez・Daniel Grimanelli・Romain Pogorelcnik・Robert A. Martienssen・大和勝幸・河内孝之・平山隆志・Olivier Mathieu
    • Organizer
      日本エピジェネティクス研究会第13回年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Analysis for transcriptional regulation mechanism of transposon-like elements in plants2019

    • Author(s)
      Ikeda, Y
    • Organizer
      日本エピジェネティクス研究会第13回年会奨励賞受賞講演
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Plant mobile domainを介した新規サイレンシング機2018

    • Author(s)
      池田陽子
    • Organizer
      遺伝研研究会「転移因子と宿主の相互作用による生命機能と進化」
    • Related Report
      2018 Research-status Report
    • Invited

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Published: 2018-04-23   Modified: 2023-01-30  

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