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Role of guard-cell chloroplasts in ABA-dependent stomatal closure

Research Project

Project/Area Number 18K06292
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionKyushu University

Principal Investigator

Shimazaki Ken-ichiro  九州大学, 理学研究院, 名誉教授 (00124347)

Co-Investigator(Kenkyū-buntansha) 後藤 栄治  九州大学, 農学研究院, 准教授 (90614256)
Project Period (FY) 2018-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords気孔 / アブシジン酸 / 孔辺細胞葉緑体 / 気孔閉鎖 / 活性酸素 / 孔辺細胞 / 葉緑体
Outline of Final Research Achievements

Participation of guard cell chloroplasts in ABA-induced stomatal closure has been shown in the previous report. In this study, we investigated the possible role of K+/H+ antiporter in the thylakoid membranes.We found that the mutants of kea3 lost production of reactive oxygens(ROS)in guard cells, and that the mutants did not show stomatal closure in response to ABA. Furthermore, we found that a drug nigericin, which stimulates the exchange of K+ and H+ across the thylakoid membranes, elicited both ROS production and stomatal closure. From the results, we conclude that K+/H+ antiporter(KEA3)is involved in the ABA-dependent stomatal closure.

Academic Significance and Societal Importance of the Research Achievements

孔辺細胞葉緑体は、通常、光による気孔開口に関与すると考えられている。しかし、その役割は依然として明確ではない。我々は、この葉緑体がアブシジン酸による気孔閉鎖に関与することを示す報告を行った。これは、これまでにない新規の考えで、孔辺細胞葉緑体の機能に新たな光を当てるものである。
しかし、その機構については不明の点が多く、上記の報告についても必ずしも認知されているわけではない。今回の研究成果は、そのことを確認するとともに、孔辺細胞葉緑体の新たな役割をより具体的に示すものである。これによって、その機構解明がさらに進むことが期待される。

Report

(7 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (3 results)

All 2024 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (1 results)

  • [Journal Article] Chloroplast K<sup>+</sup>/H<sup>+</sup><scp>EXCHANGE ANTIPORTER</scp> 3 modulates abscisic acid‐induced reactive oxygen species generation in guard cells2024

    • Author(s)
      Yamada Naotaka、Onjo Michio、Sonoike Kintake、Shimazaki Ken‐ichiro、Iwai Sumio
    • Journal Title

      Physiologia Plantarum

      Volume: 176 Issue: 1

    • DOI

      10.1111/ppl.14136

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure2019

    • Author(s)
      Sumio Iwai, Sho Ogata, Naotaka Yamada, Michio Onjo, Kintake Sonoike, Ken-ichiro Shimazaki
    • Journal Title

      Plant Direct

      Volume: 1 Issue: 5 Pages: 1-10

    • DOI

      10.1002/pld3.137

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] アブシジン酸誘導性気孔閉鎖における光合成電子伝達系の重要性2018

    • Author(s)
      山田 直隆、岩井 純夫、緒方 翔、新北 大樹、遠城 道雄、園池 公毅、島崎 研一郎
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2025-01-30  

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