| Project/Area Number |
18K06295
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 44030:Plant molecular biology and physiology-related
|
|---|
| Research Institution | Kanagawa University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2018-04-01 – 2023-03-31
|
| Project Status |
Completed (Fiscal Year 2022)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 光合成電子伝達 / シトクロム / 紅色細菌 / 遺伝子操作 / 紅色光合成細菌 / クライオ電子顕微鏡 / 光合成 / 電子伝達 / シトクロムbc複合体 / チトクロムbc複合体 |
|---|
| Outline of Final Research Achievements |
Cytochrome bc1 complex has been considered as an essential component in photosynthetic electron transport processes. However, it was first shown in this study that a mutant of purple bacteria lacking the genes coding this complex is capable of photosynthetic growth ability. This suggests a presence of novel enzymes complementing the function of the cytochrome bc1 which oxidizes quinols and reduces soluble electron carriers. Although such novel enzymes could not be isolated, a soluble protein reduced by these novel enzymes and working as an electron donor to the photochemical reaction center was identified as a mono-heme cytochrome c which natural function is reduction of nitrous oxygen. And, a process of transferring the quinol molecules derived from the photochemical reaction centers to the cytochrome bc1 complex was successfully visualized.
|
| Academic Significance and Societal Importance of the Research Achievements |
光合成電子伝達という、よく研究されその構成要素も固定されてきた反応系が、別の構成要素を利用し別の電子伝達反応系と動的にリンクしうることが分かったことが最大の成果である。加えてその過程の一部を可視化できたことも学術的に大きな成果であると言える。これらの成果を活用し、シトクロム等の構成要素の欠落や導入、あるいは部位特異的変異の導入により、光合成の電子の流れを人為的に変え、有用物質の大量合成や環境浄化に利用できるようになると期待できる。
|
