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Decoding symbiosis signal encoded by calcium oscillation

Research Project

Project/Area Number 18K06299
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionKwansei Gakuin University

Principal Investigator

Takeda Naoya  関西学院大学, 生命環境学部, 准教授 (60571081)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords根粒共生 / 植物微生物間相互作用 / カルシウムシグナリング / 菌根共生 / 共生 / 植物微生物相互作用 / 根粒菌 / アーバスキュラー菌根菌 / 菌根菌
Outline of Final Research Achievements

In mutualistic plant-microbe interactions; arbuscular mycorrhizal (AM) symbiosis and rhizobial symbiosis, we analyzed "calcium spiking," which are periodic oscillations in intracellular Ca2+ concentration induced by the acceptance of symbiotic signaling molecules in the host plant. Transcriptome analysis in root hair cells, the site of the first interaction with the symbionts, identified symbiotic genes under the control of calcium oscillation. From their functional analysis, we predicted the roles of calcium spiking encoded by the oscillation phenomenon of Ca2+ which may give Ca2+, a general second messenger, characteristics of a symbiotic signaling. Furthermore, we discovered and analyzed a symbiotic signal receptor mutant that lead to the elucidation of the mechanism of calcium oscillation generation.

Academic Significance and Societal Importance of the Research Achievements

共生応答としてのカルシウム振動の他にも、植物細胞内では根毛細胞の生長や分岐・膨張などの形態異常時、花粉管伸長や気孔開閉に関連して、異なる波形のCa2+のオシレーションが発生している。本研究は、このような共生以外のCa2+濃度変動が関与する生理現象の解析に拡張することが可能である。また、現在、トランスクリプトーム解析などから得られた知見から、低環境負荷型の農業技術として期待される「微生物肥料」としての共生能の利用に向けた研究に取り組み、特許などの成果を挙げている。そのため、本研究による成果も共生能の向上と制御を行う技術開発に結び付けることが可能である。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (12 results)

All 2022 2021 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 6 results)

  • [Journal Article] Endogenous gibberellins affect root nodule symbiosis via transcriptional regulation of NODULE INCEPTION in Lotus japonicus2021

    • Author(s)
      Akamatsu Akira、Nagae Miwa、Nishimura Yuka、Romero Montero Daniela、Ninomiya Satsuki、Kojima Mikiko、Takebayashi Yumiko、Sakakibara Hitoshi、Kawaguchi Masayoshi、Takeda Naoya
    • Journal Title

      The Plant Journal

      Volume: 105 Issue: 6 Pages: 1507-1520

    • DOI

      10.1111/tpj.15128

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Gibberellin Promotes Fungal Entry and Colonization during Paris-Type Arbuscular Mycorrhizal Symbiosis in Eustoma grandiflorum2019

    • Author(s)
      Tominaga Takaya、Miura Chihiro、Takeda Naoya、Kanno Yuri、Takemura Yoshihiro、Seo Mitsunori、Yamato Masahide、Kaminaka Hironori
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 3 Pages: 565-575

    • DOI

      10.1093/pcp/pcz222

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] LACK OF SYMBIONT ACCOMMODATION controls intracellular symbiont accommodation in root nodule and arbuscular mycorrhizal symbiosis in Lotus japonicus2019

    • Author(s)
      Suzaki Takuya、Takeda Naoya、Nishida Hanna、Hoshino Motomi、Ito Momoyo、Misawa Fumika、Handa Yoshihiro、Miura Kenji、Kawaguchi Masayoshi
    • Journal Title

      PLOS Genetics

      Volume: 15 Issue: 1 Pages: e1007865-e1007865

    • DOI

      10.1371/journal.pgen.1007865

    • NAID

      120007128215

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] フォスファチジルイノシトール輸送タンパク質が調整する根粒菌感染機構の解明2022

    • Author(s)
      赤松明,武田直也
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Relationship between the plant cell wall and the infection process of symbiotic microbes, focusing on COBRA genes in Lotus japonicus2021

    • Author(s)
      Romero-Montero Daniela, Mayu Kawasaki , Akira Akamatsu, Naoya Takeda
    • Organizer
      Plant Cell Wall Biology 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sterol acyltransferase is involved in the regulation of root nodule symbiosis2021

    • Author(s)
      Akihiro Yamazaki, Yozo Okazaki, Yasuhiro Higashi, Kazuki Saito, Akira Akamatsu, Naoya Takeda, Akira Miyahara, Miwa Nagae, Yosuke Umehara, Makoto Hayashi
    • Organizer
      日本植物生理学会
    • Related Report
      2020 Research-status Report
  • [Presentation] Characterization of cis-Regulatory Regions in NIN Promoter Required for Infection Thread Formation and Gibberellin Responses2019

    • Author(s)
      Akamatsu A, Nagae M, Nishimura Y, Kawaguchi M, Takeda N
    • Organizer
      Internatinal conference of Nitrogen fixation
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characterization of a cis-regulatory region in NIN promoter required for infection thread formation in Lotus japonicus2019

    • Author(s)
      Akira Akamatsu, Miwa Nagae, Yuka Nishimura, Naoya Takeda
    • Organizer
      IS-MPMI XVIII Congress
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Gibberellin promotes fungal entry and colonization during Paris-type arbuscular mycorrhizal symbiosis in Eustoma grandiflorum2019

    • Author(s)
      Takaya Tominaga, Chihiro Miura, Naoya Takeda, Yuri kanno, Yoshihiro Takemura, Mitsunori Seo, Masahide Yamato, Hironori Kaminaka
    • Organizer
      IS-MPMI XVIII Congress
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 根毛1組織のトランスクリプトーム解析による表皮特異的共生遺伝子の探索2019

    • Author(s)
      川崎真優 赤松明 米倉円佳 近藤聡 武田直也
    • Organizer
      植物微生物研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] Gibberellin regulates root nodule symbiosis through autoregulation of nodulation system2018

    • Author(s)
      Akira Akamatsu, Miwa Nagae, Yuka Nishimura, Masayoshi Kawaguchi, Naoya Takeda
    • Organizer
      13th European Nitrogen Fixation Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Gibberellin regulates root nodule symbiosis through autoregulation2018

    • Author(s)
      Naoya Takeda
    • Organizer
      Satellite Meeting on Signal Transduction Pathways in Nitrogen-Fixing Symbioses
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2024-12-25  

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