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Studies on diel changes in metabolic pathways for safe cell growth and division in photosynthetic eukaryotes

Research Project

Project/Area Number 18K06300
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionNational Institute of Genetics

Principal Investigator

Fujiwara Takayuki  国立遺伝学研究所, 遺伝形質研究系, 助教 (10595151)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords細胞周期 / 日周 / 真核光合成生物 / 単細胞紅藻 / トランスクリプトーム / 遺伝的改変 / モデル藻類 / 単細胞藻類 / 光合成 / 藻類 / 代謝 / CDK / 紅藻 / 昼夜リズム / 真核藻類 / Cyanidioscyzon merolae / 日周リズム / 好気エネルギー変換 / 細胞増殖
Outline of Final Research Achievements

Photosynthetic eukaryotes have enabled highly efficient energy conversion through the acquisition of chloroplasts. However, if the supply of substrates and utilization of its products in photosynthesis (metabolic flow) are not rotated properly, reactive oxygen species are generated from the photosystem and damage the host cell. In this study, we produced the cell cycle mutants of unicellular red algae and conducted transcriptome analysis in order to clearly assess and understand the effects of diurnal and cell cycle on metabolism. The results showed that many major metabolic pathways fluctuate diurnally in a cell cycle-independent manner, while only limited metabolic pathways, such as dNTP synthesis, depend on the cell cycle. This gene expression information will lead to the elucidation of the mechanism for the safe growth of photosynthetic eukaryotes.

Academic Significance and Societal Importance of the Research Achievements

真核藻類は、細胞が分化しないため、同一の細胞で光合成と細胞分裂を行う。また、陸上植物より早く分岐したため、一次植物が成立した当初の特徴をより残している。したがって、植物の祖先細胞が、葉緑体を得て光合成を行うために、どのような生理的・代謝的な基盤を発達させたかを知る手がかりを得やすい。本研究では、真核藻類において、光合成、代謝および細胞周期(生長と分裂)の関係を調べるための遺伝子発現データベースを構築した。この情報は、上記の問題の解明だけでなく、近年再生可能エネルギーの原料として注目される藻類のバイオマス生産の向上のためにも有用である。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (10 results)

All 2020 2019 2018

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 6 results) Presentation (2 results) (of which Invited: 1 results)

  • [Journal Article] An Inducible and Repressible Gene Expression System in the Unicellular Red Alga <i>Cyanidioschyzon merolae</i>2020

    • Author(s)
      Miyagishima Shin-ya、Fujiwara Takayuki
    • Journal Title

      CYTOLOGIA

      Volume: 85 Issue: 2 Pages: 91-92

    • DOI

      10.1508/cytologia.85.91

    • NAID

      130007864044

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2020-06-25
    • Related Report
      2020 Annual Research Report
  • [Journal Article] Mitotic Karyotype of the Primitive Red Alga <i>Cyanidioschyzon merolae</i> 10D2020

    • Author(s)
      T. Kuroiwa, F. Yagisawa, T. Fujiwara, Y. Inui, T.M. Matsunaga, S. Katoi, S. Matsunaga, N. Nagata, Y. Imoto, H. Kuroiwa
    • Journal Title

      CYTOLOGIA

      Volume: 85 Issue: 2 Pages: 107-113

    • DOI

      10.1508/cytologia.85.107

    • NAID

      130007864023

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2020-06-25
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between Cell Cycle and Diel Transcriptomic Changes in Metabolism in a Unicellular Red Alga2020

    • Author(s)
      Fujiwara Takayuki、Hirooka Shunsuke、Ohbayashi Ryudo、Onuma Ryo、Miyagishima Shin-ya
    • Journal Title

      Plant Physiology

      Volume: 183 Issue: 4 Pages: 1484-1501

    • DOI

      10.1104/pp.20.00469

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficient open cultivation of cyanidialean red algae in acidified seawater2020

    • Author(s)
      Hirooka Shunsuke、Tomita Reiko、Fujiwara Takayuki、Ohnuma Mio、Kuroiwa Haruko、Kuroiwa Tsuneyoshi、Miyagishima Shin-ya
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 1-12

    • DOI

      10.1038/s41598-020-70398-z

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evolutionary Changes in DnaA-Dependent Chromosomal Replication in Cyanobacteria2020

    • Author(s)
      Ohbayashi Ryudo、Hirooka Shunsuke、Onuma Ryo、Kanesaki Yu、Hirose Yuu、Kobayashi Yusuke、Fujiwara Takayuki、Furusawa Chikara、Miyagishima Shin-ya
    • Journal Title

      Frontiers in Microbiology

      Volume: 11 Pages: 786-786

    • DOI

      10.3389/fmicb.2020.00786

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ESCRT machinery mediates cytokinetic abscission in the unicellular red alga Cyanidioschyzon merolae.2020

    • Author(s)
      Yagisawa F., Fujiwara T., Takemura T., Kobayashi Y., Sumiya N., Miyagishima S. Y., Nakamura S., Imoto Y., Misumi O., Tanaka K., Kuroiwa H., Kuroiwa T.
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 8 Pages: 169-169

    • DOI

      10.3389/fcell.2020.00169

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Integration of a Galdieria plasma membrane sugar transporter enables heterotrophic growth of the obligate photoautotrophic red alga Cynanidioschyzon merolae2019

    • Author(s)
      Fujiwara Takayuki、Hirooka Shunsuke、Mukai Mizuna、Ohbayashi Ryudo、kanesaki Yu、Watanabe Satoru、Miyagishima Shin-ya
    • Journal Title

      Plant Direct

      Volume: 3 Issue: 4

    • DOI

      10.1002/pld3.134

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Coordination of Polyploid Chromosome Replication With Cell Size and Growth in a Cyanobacterium.2019

    • Author(s)
      Ohbayashi R, Nakamachi A, Hatakeyama TS, Watanabe S, Kanesaki Y, Chibazakura T, Yoshikawa H, Miyagishima SY.
    • Journal Title

      mBio

      Volume: 10 Issue: 2

    • DOI

      10.1128/mbio.00510-19

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 単細胞紅藻シアニディオシゾンにおける細胞分裂制御リズムの翻訳非依存性2020

    • Author(s)
      藤原崇之, 宮城島進也
    • Organizer
      第27回日本時間生物学会学術大会 2020年9月27日
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 真核藻類の日周における代謝と細胞周期の時間分業2018

    • Author(s)
      藤原崇之, 廣岡俊亮, 大林龍胆, 宮城島進也
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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