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Analysis of translation efficiency and translation initiation site in plant light response

Research Project

Project/Area Number 18K06304
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Kurihara Yukio  国立研究開発法人理化学研究所, 環境資源科学研究センター, 上級研究員 (60455342)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords光応答 / 翻訳効率 / 翻訳開始点 / シロイヌナズナ / 転写開始点 / リボソームプロファイリング / 翻訳変化 / リボソーム
Outline of Final Research Achievements

After germination, plants perceive light and dramatically change their gene expression profile. Blue light is required for regulation of hypocotyl elongation. This research aimed to reveal changes of translation efficiency and translation initiation site, which occur when dark-grown young Arabidopsis seedlings are exposed to blue light. For the purpose, ribosome profiling analysis was performed. The results showed that more than 35,000 ORFs were translated in the light response and translation efficiency of 18% of them was significantly altered by blue light exposure. In addition, the results identified some genes in which translation initiation sites changed accompanied with change of transcription start sites.

Academic Significance and Societal Importance of the Research Achievements

これまでの学術研究においては、mRNAの蓄積の高低が遺伝子の発現量の指標として用いられることが多かった。本研究は、遺伝子発現を単にmRNAの量ではなく、翻訳に向けたmRNAの質という概念を加えて発展的に探るものである。したがって、本研究で得られる成果は、これからの遺伝子発現から生命現象を理解する研究全体に対して一石を投じるものとなるはずである。また本研究は、植物の環境応答に応じた遺伝子発現の調節機構の新たな一面を明らかにするものでもある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2020 2019 2018

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Time-Course Transcriptome Study Reveals Mode of bZIP Transcription Factors on Light Exposure in Arabidopsis2020

    • Author(s)
      Kurihara Yukio、Makita Yuko、Shimohira Haruka、Matsui Minami
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 6 Pages: 1993-1993

    • DOI

      10.3390/ijms21061993

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Translational Landscape of Protein-Coding and Non-Protein-Coding RNAs upon Light Exposure in Arabidopsis2019

    • Author(s)
      Kurihara Yukio、Makita Yuko、Shimohira Haruka、Fujita Tomoya、Iwasaki Shintaro、Matsui Minami
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 3 Pages: 536-545

    • DOI

      10.1093/pcp/pcz219

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] RNA-Mediated Plant Behavior2019

    • Author(s)
      Ohtani Misato、Kurihara Yukio、Seki Motoaki、Crespi Martin
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 9 Pages: 1893-1896

    • DOI

      10.1093/pcp/pcz168

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The plant translatome surveyed by ribosome profiling2019

    • Author(s)
      Fujita Tomoya, Kurihara Yukio, Iwasaki Shintaro
    • Journal Title

      Plant And Cell Physiology

      Volume: 印刷中 Issue: 9 Pages: 1917-1926

    • DOI

      10.1093/pcp/pcz059

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transcripts from downstream alternative transcription start sites evade uORF-mediated inhibition of gene expression in Arabidopsis2018

    • Author(s)
      Kurihara Yukio、Makita Yuko、Kawashima Mika、Fujita Tomoya、Iwasaki Shintaro、Matsui Minami
    • Journal Title

      Proceedings of the National Academy of Sciences USA

      Volume: 115 Issue: 30 Pages: 7831-7836

    • DOI

      10.1073/pnas.1804971115

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] シロイヌナズナの青光応答時におけるリボソームプロファイリング解析2020

    • Author(s)
      栗原志夫,蒔田由布子,下平春花,藤田智也,岩崎信太郎,松井南
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] シロイヌナズナ幼苗の青色光への応答時におけるORFの翻訳変化の解明2019

    • Author(s)
      栗原志夫、蒔田由布子、下平春花、藤田智也、岩崎信太郎、松井南
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 植物は光照射が起こす転写開始点の位置変化によってuORF による遺伝子発現抑制を免れる2019

    • Author(s)
      栗原 志夫、蒔田由布子、川島美香、藤田智也、岩崎信太郎、松井南
    • Organizer
      第21回日本RNA 学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Transcription start site-dependent fine-tuning of gene expression during light exposure in Arabidopsis.2019

    • Author(s)
      Kurihara Y, Makita Y, Kawashima M, Fujita T, Iwasaki S, Matsui M
    • Organizer
      Post-transcriptional Gene Regulation in Plants 2019 NARA
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] シロイヌナズナの光応答時におけるタンパク質をコードしないORFの翻訳変化の解明2019

    • Author(s)
      栗原志夫、蒔田由布子、下平春花、藤田智也、岩崎信太郎、松井南
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 植物は光受容によって起こる転写開始点の位置変化によってuORFによる遺伝子発現抑制を免れる2018

    • Author(s)
      栗原志夫、蒔田由布子、川島美香、藤田智也、岩崎信太郎、松井南
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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