| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
To elucidate the maintenance mechanism of spindle bipolarity in plant cells, we analyzed microtubule dynamics in the minispindle. Our results suggested that kinetochore microtubules serve as scaffolds for microtubule nucleation and that newly formed microtubules bundle kinetochore microtubules. In addition, we analyzed the cell division frequency and spindle morphology of tobacco BY-2 cells and Coleochaete cultured under hypergravity or simulated microgravity to elucidate the effects of gravity environments different from 1G on spindle formation and cell division in plants. Our results showed that the simulated microgravity promoted cell division in plant cells and suggested the possibility that the gravity environment different from 1G may not affect the spindle formation.
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