| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Physarum polycephalum has large mitochondrial nucleoids, which are mitochondrial DNA (mtDNA)-protein complexes. In this study, we developed a semi-in vitro assay to detect DNase activity by microscopic observation of the digestion of mtDNA in isolated mitochondria from P. polycephalum and the DNase involved in maternal inheritance was characterized. When the mitochondria isolated from zygotes were used, the digestion of mtDNA in isolated mitochondria was specifically observed in the presence of Mg2+ and maximum activity was detected at pH 7.7. During the digestion of mtDNA, mitochondrial membrane integrity was maintained in vivo. Furthermore, we also developed a method to distinguish uniparental mitochondria in zygotes of P. polycephalum by chemical staining. Our results showed that the proliferation of maternal mitochondria rapidly occurred after the initiation of digestion of paternal mtDNA.
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