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Study of morphogenesis regulated by the Wnt/PCP pathway during early mouse embryogenesis

Research Project

Project/Area Number 18K06324
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44040:Morphology and anatomical structure-related
Research InstitutionNational Institute of Genetics

Principal Investigator

Ajima Rieko  国立遺伝学研究所, 遺伝形質研究系, 助教 (10615066)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsWnt5a / 平面内極性 / 前後軸伸長 / 形態形成 / マウス初期発生 / 非古典的Wnt / PCP / 結合因子同定 / 結合因子
Outline of Final Research Achievements

In vertebrate development, the Wnt/PCP signaling pathway regulates the morphology of various tissues by controlling two events: tissue elongation and planar cell polarity establishment. However, the molecular mechanism underlying these events remain unclear. In this research project, we identified multiple factors that change the binding to PCP core proteins Pk and Dvl depending on the presence or absence of Wnt5a stimulation. I established gene targeted mice for the obtained proteins, and analyzed the phenotypes in anterior-posterior axis elongation and planar cell polarity establishment in the node. As a result, we identified several factors that play important roles only in planar cell polarity establishment. This indicates that tissue elongation and planar cell polarity establishment are controlled by different factors downstream of the Wnt/PCP signaling pathway.

Academic Significance and Societal Importance of the Research Achievements

Wnt/PCPシグナル経路は、様々な組織の形態形成および 恒常性の維持を制御しており、その異常は遺伝疾患の原因になることが知られている。本研究で同定されたWnt5a下流因子の各組織における機能を調べることは、遺伝疾患の発祥機序の解明につながると考えられる。また、再生医療の分野において、多能性細胞から分化誘導した組織が生体内で機能する上で、組織の形態と極性の制御は必須である。本研究で得られた結果は、再生医療の分野にも重要な知見をもたらすと考えられる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2021 2020 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Journal Article] Placental defects lead to embryonic lethality in mice lacking the Formin and PCP proteins Daam1 and Daam22020

    • Author(s)
      Nakaya Masa-aki、Gudmundsson Kristibjorn Orri、Komiya Yuko、Keller Jonathan R.、Habas Raymond、Yamaguchi Terry P.、Ajima Rieko
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 4 Pages: e0232025-e0232025

    • DOI

      10.1371/journal.pone.0232025

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Identification and functional analysis of Wnt5a downstream targets during early development in mouse2021

    • Author(s)
      安島 理恵子
    • Organizer
      Wnt研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Identification and characterization of Wnt5a downstream targets during early development in mouse2021

    • Author(s)
      Rieko Ajima
    • Organizer
      53rd JSDB meeting
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] Identification and characterization of Wnt5a downstream targets during early development in mouse2021

    • Author(s)
      Rieko Ajima
    • Organizer
      JSDB Online Trial meeting 2020
    • Related Report
      2020 Research-status Report
  • [Presentation] Identification of Wnt5a downstream targets during early development in mouse2019

    • Author(s)
      Rieko Ajima
    • Organizer
      The 52nd JSDB meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Identification of Wnt5a downstream targets during early development in mouse2019

    • Author(s)
      Rieko Ajima
    • Organizer
      Wnt Signaling Gordon Research Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Identification of Wnt5a downstream targets during early development in mouse2019

    • Author(s)
      Rieko Ajima
    • Organizer
      CDBL seminar series, NCI-Frederick, NIH
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Identification of Wnt5a downstream targets during early development in mouse2019

    • Author(s)
      Rieko Ajima
    • Organizer
      Annual Meeting of Japanese Society of Developmental Biologists
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Identification of Wnt5a downstream targets during early development in mouse2018

    • Author(s)
      Rieko Ajima
    • Organizer
      Joint Annual Meeting of JSDB and JSCB
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2023-01-30  

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