| Project/Area Number |
18K06330
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44050:Animal physiological chemistry, physiology and behavioral biology-related
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| Research Institution | The Graduate University for Advanced Studies |
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Principal Investigator |
Satoh Aya 総合研究大学院大学, 先導科学研究科, 連携研究員 (60378560)
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| Co-Investigator(Kenkyū-buntansha) |
寺井 洋平 総合研究大学院大学, 先導科学研究科, 助教 (30432016)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 体内時計 / 概潮汐時計 / 時計遺伝子 / マングローブ / コオロギ / RNA干渉法 / RNA-seq / 潮汐サイクル / 潮間帯 / 潮汐 / 概潮汐リズム |
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| Outline of Final Research Achievements |
Mangrove crickets Apteronemobius asahinai are endemic to mangrove forest floors. They show circatidal rhythmicity with the period of 12.6 hours in their locomotor activity under constant condition. Little is known about the molecular mechanisms underlying the circatidal clock. To identify circatidal clock genes, we investigated rhythmic gene expression under constant darkness by RNA-seq. We identified higher number of transcripts with circatidal rhythmicity (284) than those with circadian (206). We also determined a high quality draft genome sequence of mangrove crickets. These dataset will facilitate further investigation of circatidal clock genes.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では，これまでどの生物においても特定されていない概潮汐時計遺伝子を解明するため，恒常条件下で発現量が概潮汐リズムを示す遺伝子群を特定できた。また，分子基盤解明の重要な情報となるゲノム配列を高精度に決定することができた。これらの研究成果は，概潮汐時計の分子基盤の今後の解明に大きく貢献すると期待される。
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