| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
To understand the molecular mechanisms of the FMRFamide-gated Na+ channel (FaNaC), we focused on the extracellular domain of the channel which must have peptide binding site(s) as well as the linking structure for the activation of the transmembrane pore. There are seven SS-bonds in the extracellular domain of FaNaC. We tested the function of each SS-bond one by one and found that the SS-bonds which are necessary to maintain the thumb domain of FaNaC are indispensable for the normal function of the channel. We next mutated several residues in the finger domain and found that several aromatic residues are important for the activation of the channel. We also made the 3D-model of FaNaC and carried out the docking simulation of FMRFamide. The results are consistent with the results of mutagenic experiments, suggesting that some of the aromatic residues are determinants of the FMRFamide binding site.
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