Isolation of novel temperature signaling molecules using a high-throughput system
| Project/Area Number |
18K06344
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44050:Animal physiological chemistry, physiology and behavioral biology-related
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| Research Institution | Konan University |
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Principal Investigator |
Ohta Akane 甲南大学, 自然科学研究科, 特別研究員 (50410717)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 温度情報伝達分子 / 温度受容体 / 低温耐性 / 温度受容ニューロン / 線虫 / C. elegans / 温度受容 / ハイスループット |
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| Outline of Final Research Achievements |
In order to identify novel genes involved in temperature signaling, genetic screening was performed using the phenotype of cold tolerance and acclimation in C. elegans. As a result, several molecules required for cold tolerance were isolated. Among them, the TRPV channels OSM-9 and OCR-2 also functioned as temperature receptors in the novel temperature-sensing neuron ADL. Furthermore, the DEG / ENaC type mechanoreceptor DEG-1 functioned as a temperature receptor in the novel temperature-sensing neuron ASG.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の線虫C. elegansの低温耐性の解析から、DEG/ENaC型のメカノ受容体(DEG-1)が温度受容体として機能することが初めて見つかった。さらに、DEG-1のヒトホモログであるMDEG2も温度受容体であることが示唆された。今後、MDEG2に対する創薬への期待が高まる可能性がある。TRPVチャネルであるOSM-9/OCR-2複合体が温度受容体として機能していたが、その温度応答性は弱かったため、OSM-9とOCR-2以外の温度受容体の存在が示唆された。
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Report
(5 results)
Research Products
(63 results)
