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Exploring the mechanism for complex petal morphogenesis: integration of the proximate and ultimate factors

Research Project

Project/Area Number 18K06366
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 45020:Evolutionary biology-related
Research InstitutionKyoto Prefectural University

Principal Investigator

Takeda Seiji  京都府立大学, 生命環境科学研究科, 准教授 (90508053)

Co-Investigator(Kenkyū-buntansha) 末次 健司  神戸大学, 理学研究科, 准教授 (70748839)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsサギソウ / MADS転写因子 / 鋸歯 / 訪花昆虫 / スズメガ / 唇弁 / TCP / 結実 / トランスクリプトーム / 花弁 / ラン / ポリネーター / 共進化
Outline of Final Research Achievements

We investigated the morphology of the labellum in the white egret flower from viewpoints of (1) the genetic and cellular mechanisms of morphogenesis and (2) the co-evolution with pollinators. By comprehensive transcriptome analysis with RNA-sequencing, we identified transcription factors expressed in the labellum. We found that early cell division and later polar cell elongation were important during formation of labellum serration. The hawkmoth visited flowers and sucked honey with hovering but not landing on the flowers, and that elimination of the serration from labellum affected the fruit set and seed weight.

Academic Significance and Societal Importance of the Research Achievements

サギソウは日本に自生する野生ランで、準絶滅危惧種に指定されている。3枚の花弁のうち「唇弁」と呼ばれる1枚は、鳥が飛んでいるような特徴的な形になる。本研究により、唇弁の形には地域差があり、DNAレベルでも少しずつ違いがあることが分かり、地域の自生種の保全が重要であることが示された。唇弁の複雑な形態は、訪花昆虫の「目印」になっており、その形態は唇弁で特異的に働く遺伝子(転写因子)によって行われることが分かった。本研究成果は、各地の自生地の特徴づけ、および特徴的な花弁形態を生みだす技術に応用可能である。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (4 results)

All 2021 2018 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Journal Article] Morphological and genetic diversities of Habenaria radiata (Orchidaceae) in Kinki area, Japan2021

    • Author(s)
      Tsutomu Tachibana, Yuki Nishikawa, Nakao Kubo, Seiji Takeda
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 1 Pages: 311-311

    • DOI

      10.3390/ijms22010311

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] サギソウの花形態形成に関する遺伝子発現解析2018

    • Author(s)
      西川友貴、立花耕、坂本智昭、木村成介、武田征士
    • Organizer
      日本植物学会 第82回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Morphological and genetic analysis for revealing petal development of Habenaria radiata2018

    • Author(s)
      Yuki Nishikawa, Tsutomu Tachibana, Tomoaki Sakamoto, Seisuke Kimura, Seiji Takeda
    • Organizer
      Basic and applied studies of plant natural products for agriculture and human health
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Remarks] サギソウの多様性調査の論文が掲載されました

    • URL

      https://kpu-als.jp/w/research/achievement/1908/

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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