• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Unravelling the genetics of leaf formation in unifoliates

Research Project

Project/Area Number 18K06375
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 45030:Biodiversity and systematics-related
Research InstitutionKanagawa University (2019-2022)
Tokyo Gakugei University (2018)

Principal Investigator

Nishii Kanae  神奈川大学, 付置研究所, 研究員 (50743770)

Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords次世代シーケンサー / 高分子DNA抽出法 / ゲノムアッセンブリ / ゲノムワイド関連解析 / 重イオンビーム照射変異体 / イワタバコ科 / 葉発生 / 茎頂分裂組織 / ゲノム / 次世代シーケンス / 次世代シークエンス / QTL解析 / ロングリードシーケンス
Outline of Final Research Achievements

Plant morphological diversity linked to their meristem activities. This project ultimately aims to reveal the evolution and diversity of plant meristems and their diversified functions. We use the genus Streptocarpus as the study material that exhibits the unique leaf meristem activity instead of the conventional shoot apical meristem. Next generation sequencing method was heavily employed in the project to perform the genetic studies in Streptocarpus, including the developments of the high molecular weight DNA extraction methods for the genome sequencing. We also performed the heavy-ion irradiation and successfully generated the mutant population. These resources are important for future genetic studies of the plants.

Academic Significance and Societal Importance of the Research Achievements

本研究は、植物の基本型から逸脱した非モデル植物に着目し、植物独自に成立した茎頂分裂組織機能の機能とその多様化機構を探ろうとした。これまで、シロイヌナズナなどのモデル植物の解析により、茎頂分裂組織の機能と分子生物学的機構が明らかとなってきた。一方、植物では茎頂分裂組織の機能が多様化し、形態多様性を生み出した一因となっている。ストレプトカーパス属はこのような植物の一つであり、本研究では、次世代シーケンサー技術を最大限に活用、また変異体集団を作成し、ストレプトカーパスの遺伝学的研究の基盤を作成した。これにより、ストレプトカーパスにおける茎頂分裂組織多様化の遺伝学的背景の探索を可能とした。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (33 results)

All 2023 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (6 results) Journal Article (14 results) (of which Int'l Joint Research: 13 results,  Peer Reviewed: 12 results,  Open Access: 8 results) Presentation (10 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (3 results)

  • [Int'l Joint Research] Royal Botanic Garden Edinburgh/The University of Edinbrugh(英国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Royal Botanic Garden Edinburgh/University of Edinburgh(英国)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] Cons. Jard. bot. Geneve(スイス)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] エジンバラ植物園(英国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] エジンバラ植物園/エジンバラ大学(英国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] エジンバラ植物園/エジンバラ大学(英国)

    • Related Report
      2018 Research-status Report
  • [Journal Article] The first genome for the Cape Primrose Streptocarpus rexii (Gesneriaceae), a model plant for studying meristem‐driven shoot diversity2022

    • Author(s)
      Nishii Kanae、Hart Michelle、Kelso Nathan、Barber Sadie、Chen Yun‐Yu、Thomson Marian、Trivedi Urmi、Twyford Alex D.、M?ller Michael
    • Journal Title

      Plant Direct

      Volume: 6 Issue: 4

    • DOI

      10.1002/pld3.388

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High Molecular Weight DNA extraction for long-read sequencing v.1 v12022

    • Author(s)
      Nishii Kanae、Moeller Michael、Hart Michelle
    • Journal Title

      protocols.io

      Volume: na Pages: 35215-35215

    • DOI

      10.17504/protocols.io.bempjc5n

    • Related Report
      2022 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Plant contig clustering based assembly (PLCL) pipeline: an efficient long-read assembly tool for plant chloroplast and mitochondrial genomes v12022

    • Author(s)
      Nishii Kanae
    • Journal Title

      protocols.io

      Volume: na Pages: 53125-53125

    • DOI

      10.17504/protocols.io.bx5dpq26

    • Related Report
      2022 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Mix and match: Patchwork domain evolution of the land plant-specific Ca2+-permeable mechanosensitive channel MCA2021

    • Author(s)
      Nishii, K., Moeller, M., and Iida, H.
    • Journal Title

      PLoS ONE

      Volume: 16 Issue: 4 Pages: 1-20

    • DOI

      10.1371/journal.pone.0249735

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Phylogenomics of Gesneriaceae using targeted capture of nuclear genes2021

    • Author(s)
      Ogutcen Ezgi、Christe Camille、Nishii Kanae、Salamin Nicolas、Moeller Michael、Perret Mathieu
    • Journal Title

      Molecular Phylogenetics and Evolution

      Volume: 157 Pages: 107068-107068

    • DOI

      10.1016/j.ympev.2021.107068

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Virus-induced gene silencing in Streptocarpus rexii (Gesneriaceae)2020

    • Author(s)
      Nishii K, Fei Y, Hudson A, Moeller M, Molnar A
    • Journal Title

      Molecular Biotechnology

      Volume: - Issue: 6-7 Pages: 317-325

    • DOI

      10.1007/s12033-020-00248-w

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] QTL dissection of floral traits in Streptocarpus (Gesneriaceae)2020

    • Author(s)
      Chen Yun-Yu、Nishii Kanae、Kidner Catherine、Hackett Christine Anne、Moeller Michael
    • Journal Title

      Euphytica

      Volume: 216 Issue: 7 Pages: 110-110

    • DOI

      10.1007/s10681-020-02647-1

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Hormonal crosstalk in the regulation of meristem activity and the phyllomorph architecture in Streptocarpus (Gesneriaceae): a review2020

    • Author(s)
      K. Nishii、A. Spada、Moeller M.
    • Journal Title

      Rheedea

      Volume: 30 Issue: 1 Pages: 96-115

    • DOI

      10.22244/rheedea.2020.30.01.04

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A molecular phylogeny of Southeast Asian Cyrtandra (Gesneriaceae) supports an emerging paradigm for Malesian plant biogeography2020

    • Author(s)
      Atkins H, Bramley G, Johnson M, Kartonegoro A, Nishii K, Kokubugata G, Moeller M, Hughes M
    • Journal Title

      Frontiers of Biogeography

      Volume: 12 Issue: 1

    • DOI

      10.21425/f5fbg44184

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The family placement of Cyrtandromoea2019

    • Author(s)
      Luna J、Richardson J, Nishii K, Clark J, Moeller M
    • Journal Title

      Systematic Botany

      Volume: 44 Issue: 3 Pages: 616-630

    • DOI

      10.1600/036364419x15620113920653

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Quantitative assessment of anisocotyly in Haberlea rhodopensis and Ramonda myconi2019

    • Author(s)
      Huang B-H, Nishii K, Wang C-N, Moeller M
    • Journal Title

      Edinburgh Journal of Botany

      Volume: 76 Issue: 3 Pages: 377-391

    • DOI

      10.1017/s0960428619000179

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Notes on Cyrtandra (Gesneriaceae) from Japan, Taiwan and Batan Island (Philippines)2019

    • Author(s)
      Nishii K, Kokubugata G, Moeller M, Atkins H
    • Journal Title

      Edinburgh Journal of Botany

      Volume: 76 Issue: 3 Pages: 333-344

    • DOI

      10.1017/s0960428619000106

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Tangled history of a multigene family: The evolution of ISOPENTENYLTRANSFERASE genes2018

    • Author(s)
      Kanae Nishii, Frank Wright, Yun-Yu Chen, Michael Moeller
    • Journal Title

      PLoS One

      Volume: 13 Issue: 8 Pages: e0201198-e0201198

    • DOI

      10.1371/journal.pone.0201198

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A first genetic map in the genus Streptocarpus generated with RAD sequencing based SNP markers2018

    • Author(s)
      Yun-Yu Chen, Kanae Nishii, Sadie Barber, Christine Hackett, Catherine A. Kidner, Karim Gharbi, Atsushi J. Nagano, Akitoshi Iwamoto, Michael Moeller
    • Journal Title

      South African Journal of Botany

      Volume: 117 Pages: 158-168

    • DOI

      10.1016/j.sajb.2018.05.009

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 葉分裂組織分子機構の理解に向けた重イオンビーム照射によるストレプトカーパス変異体の作成2023

    • Author(s)
      西井かなえ, Moeller Michael, Kelso Nathan, France Hazel, Barber Sadie, 林依子, 石井公太郎, 阿部知子
    • Organizer
      理研シンポジウム「重イオンビーム育種による持続可能な社会や特産品創出の実現」
    • Related Report
      2022 Annual Research Report
  • [Presentation] DNA quality impact on long-read sequencing: a case study from the recalcitrant plant Streptocarpus2022

    • Author(s)
      Nishii Kanae, Moeller Michael, Hart Michelle
    • Organizer
      Edinburgh Genomics PacBio Day
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Plant contig clustering-based genome assembly (PLCL) pipeline: assembling the chloroplast and mitochondrial genomes from Oxford Nanopore Technologies long-read sequencing2022

    • Author(s)
      Nishii Kanae, Moeller Michael
    • Organizer
      The 86th Annual Meeting of the Botanical Society of Japan
    • Related Report
      2022 Annual Research Report
  • [Presentation] High molecular weight DNA extraction protocols for non-model plant species - the first step in long-read sequencing2022

    • Author(s)
      Nishii Kanae, Forrest Laura L, Bell David, Ruhsam Markus, Hollingsworth Peter M, Howard Caroline, Todorovic Maja, Hart Michelle
    • Organizer
      The 5th European Conservation Genetics meeting
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ストレプトカーパス属の異種交配系統における減数分裂の観察2022

    • Author(s)
      西井かなえ、Moeller Michael
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Evolutionary studies of the Ca2+-permeable mechanosensitive channel MCA2021

    • Author(s)
      西井かなえ、Moeller Michael、飯田秀利
    • Organizer
      日本植物学会 第85回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ストレプトカルプス異種交配系統を用いた花形態形成因子のQTL解析2021

    • Author(s)
      西井かなえ, CHEN Yun-Yu, KIDNER Catherine, HACKETT Christine Anne, MOELLER Michael
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] ストレプトカルプス(イワタバコ科)のウィルス誘導性遺伝子サイレンシング2020

    • Author(s)
      西井かなえ, FEI Yue, HUSDON Andrew, MOELLER Michael, MOLNAR Attila
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ヨーロッパ産イワタバコ科子葉の形態形成解析2020

    • Author(s)
      西井かなえ、ファンビンホン、ワンジュンヌ、モラマイケル
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] サイトカイニン合成遺伝子ISOPENTENYLTRANSFERASEの複雑な進化過程2018

    • Author(s)
      西井かなえ, Frank Wright, Yun-Yu Chen, Michael Moeller
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
  • [Remarks] PLCL pipeline

    • URL

      https://www.protocols.io/view/plant-contig-clustering-based-assembly-plcl-pipeli-j8nlk4qw6g5r/v1

    • Related Report
      2021 Research-status Report
  • [Remarks] HMW DNA extraction for long-read sequencing

    • URL

      https://www.protocols.io/view/high-molecular-weight-dna-extraction-for-long-read-n2bvjyqxnvk5/v1

    • Related Report
      2021 Research-status Report
  • [Remarks] DNA extraction protocol for long read sequencing

    • URL

      https://stories.rbge.org.uk/archives/30792

    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi