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The study of migration of amphibians during formation of Japan islands

Research Project

Project/Area Number 18K06432
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 45040:Ecology and environment-related
Research InstitutionWaseda University

Principal Investigator

NAKAMURA MASAHISA  早稲田大学, 理工学術院総合研究所(理工学研究所), その他(招聘研究員) (40130025)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords日本列島の形成 / 陸上小型歩行動物 / ツチガエル / 国内移動経路 / 時系列分子系統樹 / ミトコンドリアDNA / 12S rRNA遺伝子 / 古地理 / 系統解析 / シーボルト / 九州の新集団 / 標準産地 / ミトコントンドリア遺伝子 / 地形変化 / 環境変化 / 陸上歩行小型動物 / ミトコンドリア12S遺伝子 / 分子系統樹 / 両生類 / 移動経路 / 分子系統解析
Outline of Final Research Achievements

The Japanese wrinkled frog Glandirana rugosa is separated into five genetically different groups. One group in western Japan is further divided into three subgroups, found in Kyushu, Shikoku, and western Honshu. We collected G. rugosa frogs at 195 sites in Japan and determined nucleotide sequences of the mitochondrial 12S and 16S rRNA genes for phylogenetic analysis. We found five local groups of frogs. In addition, we found another group in southeastern Kyushu that did not cluster with any of the pre-existing five groups of G. rugosa on the phylogenetic trees. The frogs in the new group and G. rugosa in Kyushu were designated a new species, named G. nakamurai. When a time-laps phylogenetic tree was constructed based on nucleotide sequences of mitochondrial 12S and 16S rRNA genes of G. rugosa, it showed a new migratory route of this frog during formation of Japan islands. The result would contribute to understand geographical changes during formation of Japan islands.

Academic Significance and Societal Importance of the Research Achievements

日本列島形成過程における陸上小型歩行動物の国内移動経路を明らかにした報告はない。ユーラシア大陸由来の陸上小型歩行動物の国内移動は周囲の地形によって大きな影響を受ける。本研究は日本列島形成過程における陸上小型歩行動物(カエル)の移動経路を明らかにした。これは今までに誰も成し得なかった研究成果である。カエルの移動経路によって、日本列島形成過程における地形変化を推測することができる。従って、本研究で得られた研究成果は古地理学分野のみならず、カエルが棲息できた環境も分かるため、古地理学及び環境学分野の発展に大きく貢献する。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (10 results)

All 2022 2020 2019 2018

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] A new frog species of the genus Glandirana from southeastern Kyushu, Japan (Anura Ranidae).2022

    • Author(s)
      M. Nakamura, Y. Nakamura, A. Oike, K. Tojo, T, Suzuki, E, Ito
    • Journal Title

      EC Veterinary Science

      Volume: 7.1 Pages: 11-23

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sex reversal induced by steroid hormones in Glandirana rugosa frogs.2020

    • Author(s)
      Masahisa Nakamura, Akira Oike, and Etsuro Ito
    • Journal Title

      JSM Sexual Medicine

      Volume: 4 Pages: 1056-1058

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Taxonomic identity of four groups of Glandirana rugosa (Anura, Ranidae) in Japan revealed by the complete mitochondrial genome sequence analysis.2020

    • Author(s)
      Masatake Mochizuki, Yoriko Nakamura, and Masahisa Nakamura
    • Journal Title

      Mitochondrial DNA part B: Resources

      Volume: 5 Issue: 3 Pages: 3721-3722

    • DOI

      10.1080/23802359.2020.1833772

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] A phylogenetically distinct group of Glandirana rugosa found in Kyushu, Japan2020

    • Author(s)
      Oike A, Mochizuki M, Tojo K, Matsyo T, Nakamura Y, Yasumasu S, Ito E, Arai T, Nakamura M
    • Journal Title

      Zoological Science

      Volume: 37(2) Issue: 2 Pages: 193-202

    • DOI

      10.2108/zs190007

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Melatonin is a potential drug for the prevention of bone loss during space flight2019

    • Author(s)
      Ikegame Mika、7名、Sekiguchi Toshio、43名、Suzuki Nobuo
    • Journal Title

      Journal of Pineal Research

      Volume: 67 Issue: 3 Pages: 1-13

    • DOI

      10.1111/jpi.12594

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nanos3 of the frog Rana rugosa: molecular cloning and characterization2018

    • Author(s)
      Maho Kodama, Madoka Yoshida, Masami Endo, Tohru Kobayashi, Akira Oike, Shigeki Yasumasu, and Masahisa Nakamura
    • Journal Title

      Develop Growth Differ

      Volume: 60 Issue: 2 Pages: 112-120

    • DOI

      10.1111/dgd.12421

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ツチガエルの性決定・性分化に対するエストロゲン及びエストロゲン合成酵素の役割2019

    • Author(s)
      大池 輝, 伊藤悦朗, 安増 茂樹, 中村 正久
    • Organizer
      日本動物学会第90回大阪大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Origin of sex chromosomes in six groups of Rana rugosa frogs inferred from a new sex-linked DNA marker2018

    • Author(s)
      Oike A, Watanabe K, Yasumasu S, Ito E, Nakamura M
    • Organizer
      Eighth International Symposium on Vertebrate Sex Determination
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ツチガエルにおける新しいZW集団の起源2018

    • Author(s)
      大池輝、伊藤悦朗、安増茂樹、中村正久
    • Organizer
      (社)日本動物学会関東支部大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Glandirana属に新種のカエル?2018

    • Author(s)
      大池 輝、安増茂樹、中村依子、伊藤悦郎、中村正久
    • Organizer
      (社)日本動物学会札幌大会(地震で開催地が東京に変更)
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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