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Analysis of the regulatory mechanism of motor learning using real-time FRET and fast-scan voltammetry

Research Project

Project/Area Number 18K06461
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 46010:Neuroscience-general-related
Research InstitutionUniversity of Toyama

Principal Investigator

Tabata Toshihide  富山大学, 学術研究部工学系, 教授 (80303270)

Co-Investigator(Kenkyū-buntansha) 上窪 裕二  順天堂大学, 医学部, 准教授 (80509670)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsシナプス / 可塑性 / ニューロン / 学習 / 運動 / 小脳 / 中枢神経系 / シナプス可塑性 / Gタンパク質共役型受容体 / アデノシン / ガンマ・アミノ酪酸 / 受容体 / Gタンパク質 / 記憶 / 脳 / 伝達物質 / GPCR
Outline of Final Research Achievements

Type-1 metabotropic glutamate receptor (mGluR1) expressed in cerebellar Purkinje cells serves as the trigger of synaptic plasticity underlying motor learning. Other types of G protein-coupled receptors (GPCRs)(B-type GABA receptor and adenosine A1 receptor) may modulate mGluR1 and this leads to facilitation or inhibition of the synaptic plasticity. Using FRET acceptor photobleaching and FRET sensitized emission techniques, we demonstrate that the GPCRs may exert mutual modulation by physically interacting with one another. Moreover, we developed a high precision electrochemical measurement system to measure the local concentration of adenosine, a neuromodulator that induces the inter-GPCR modulation.

Academic Significance and Societal Importance of the Research Achievements

本研究は、中枢ニューロンにおいて異なるリガンドを受容するGPCRが複合体を恒常的に形成し、直接相互作用することによって互いの機能を修飾していることを明らかにした。従来、GPCRは個別の細胞内シグナリング・カスケードを通じて細胞反応を媒介していると考えられてきた。したがって、本研究の成果は全く新しい細胞シグナリングの原理を提示するものである。また、GPCR相互作用はシナプス可塑性の誘導を調整している可能性があり、本研究で開発された神経変調因子の局所濃度測定法を用いて相互作用の解析を進めれば、学習効率の制御の仕組みを明らかにし、学習・記憶困難を伴う神経疾患の治療法の開発に道を拓くことが期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (24 results)

All 2021 2020 2019 2018 Other

All Journal Article (9 results) (of which Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (12 results) Remarks (3 results)

  • [Journal Article] A test potential booster for fast-scan cyclic voltammetry with an electrophysiological amplifier2020

    • Author(s)
      Nagai H, Yokoi T, Kano M, Tabata T
    • Journal Title

      Ayalytical Biochemistry

      Volume: 610 Pages: 113934-113934

    • DOI

      10.1016/j.ab.2020.113934

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 異種G蛋白質共役型受容体相互作用による代謝型グルタミン酸受容体の制御2020

    • Author(s)
      上窪裕二,田端俊英,坂入伯駿,櫻井隆
    • Journal Title

      脳神経内科

      Volume: -

    • Related Report
      2020 Annual Research Report
  • [Journal Article] G Protein-Coupled Glutamate and GABA Receptors Form Complexes and Mutually Modulate Their Signals2020

    • Author(s)
      Sakairi Hakushun、Kamikubo Yuji、Abe Masayoshi、Ikeda Keisuke、Ichiki Arata、Tabata Toshihide、Kano Masanobu、Sakurai Takashi
    • Journal Title

      ACS Chemical Neuroscience

      Volume: 11 Issue: 4 Pages: 567

    • DOI

      10.1021/acschemneuro.9b00599

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 分子から迫る神経薬理学:GABAB受容体の種類と構造2019

    • Author(s)
      上窪裕二、坂入伯駿、田端俊英
    • Journal Title

      Clinical Neuroscience

      Volume: 37 Pages: 504-505

    • Related Report
      2019 Research-status Report
  • [Journal Article] 分子から迫る神経薬理学:GABAB受容体の生理的機能2019

    • Author(s)
      田端俊英、上窪裕二、坂入伯駿
    • Journal Title

      Clinical Neuroscience

      Volume: 37 Pages: 630-631

    • Related Report
      2019 Research-status Report
  • [Journal Article] 分子から迫る神経薬理学:GABAB受容体の薬理と臨床応用2019

    • Author(s)
      坂入伯駿、上窪裕二、田端俊英
    • Journal Title

      Clinical Neuroscience

      Volume: 37 Pages: 760-761

    • Related Report
      2019 Research-status Report
  • [Journal Article] Monitoring of glutamate-induced excitotoxicity by mitochondrial oxygen consumption.2019

    • Author(s)
      Kumagai A, Sasaki T, Matsuoka K, Abe M, Tabata T, Itoh Y, Fuchino H, Wugangerile S, Suga M, Yamaguchi T, Kawahara H, Nagaoka Y, Kawabata K, Furue MK, Takemori H
    • Journal Title

      Synapse

      Volume: 73

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lack of modulatory effect of the SCN5A R1193Q polymorphism on cardiac fast Na+ current at body temperature.2018

    • Author(s)
      Abe M, Kinoshita K, Matsuoka K, Nakada T, Miura K, Hata Y, Nishida N, Tabata T
    • Journal Title

      PLoS One

      Volume: 13 Issue: 11 Pages: e0207437-e0207437

    • DOI

      10.1371/journal.pone.0207437

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A mutant HCN4 channel in a family with bradycardia, left bundle branch block, and left ventricular noncompaction2018

    • Author(s)
      R. Yokoyama, K. Kinoshita, Y. Hata, M. Abe, K. Matsuoka, K. Hirono, M. Kano, M. Nakazawa, F. Ichida, N. Nishida, T. Tabata
    • Journal Title

      Heart and Vessels

      Volume: 33 Issue: 7 Pages: 802

    • DOI

      10.1007/s00380-018-1116-6

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Carbon powder-filled microelectrode: an easy-to-fabricate probe for single-cell electrochemistry2021

    • Author(s)
      Tsujimura A, Kamae Y, Kawasaki H, Nagai H, Tabata T
    • Organizer
      日本神経科学大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] A simple method to fabricate a robust single-carbon fiber electrode for fast-scan cyclic voltammetry2020

    • Author(s)
      Nagai H, Yokoi T, Tabata T
    • Organizer
      日本神経科学大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 大脳皮質神経細胞の電気刺激によるPtprd遺伝子微小エクソンの選択的スプライシング調節2020

    • Author(s)
      出村 舞奈、今井 彩子、田端 俊英、森 寿、吉田 知之
    • Organizer
      中部日本生理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 1 型代謝型グルタミン酸受容体と GABAB 受容体は異種 GPCR 間複合体を形成し、互いの細胞内シグナルを双方向に制御する2020

    • Author(s)
      坂入伯駿,上窪裕二,田端俊英,櫻井隆
    • Organizer
      薬理学会関東部会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 代謝型グルタミン酸受容体と GABAB受容体の複合体形成とシグ ナルクロストーク2020

    • Author(s)
      上窪裕二,坂入伯駿,田端俊英,櫻井隆
    • Organizer
      日本神経科学大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Type 1 metabotropic glutamate receptor 1 and GABA B receptor form complexes at cell membrane and mutually modulate their function2020

    • Author(s)
      Hakushun Sakairi, Yuji Kamikubo, Toshihide Tabata, Takashi Sakurai
    • Organizer
      日本薬理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] スマートフォンアプリを用いたエピソード記憶の強化に関する研究開発2020

    • Author(s)
      土田史高、横山茜、荒子貴明、澤田詩織、青塚一樹、北村和希、田端俊英
    • Organizer
      情報処理学会全国大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Implementation and characterization of a non-linear filter to extract neurobiological signals with minimal distortion2019

    • Author(s)
      Toshihide Tabata, Rihito Nagamatsu, Masayoshi Abe, Kazuki Kitamura, Tomoki Matsui, Ryo Takemura, Hakushun Sakairi, Yuji Kamikubo
    • Organizer
      日本神経科学大会
    • Related Report
      2019 Research-status Report
  • [Presentation] エピソード記憶を維持・向上させるヘルスケアシステムの研究開発2019

    • Author(s)
      青塚一樹、北村和希、土田史高
    • Organizer
      日本医療福祉設備学会
    • Related Report
      2019 Research-status Report
  • [Presentation] “Mobile neuropsychological lab” for studying episodic memory function in business persons2019

    • Author(s)
      Kazuki Kitamura, Kazuki Aozuka, Ayane Yokoyama, Takaaki Arako, Fumitaka Tsuchida, Toshihide Tabata
    • Organizer
      Toyama Forum for Academic Sumit on “Dynamic Brain”
    • Related Report
      2019 Research-status Report
  • [Presentation] Functional interaction and Complex formation of metabotropic glutamate and GABA receptor2019

    • Author(s)
      Sakairi H, Kamokubo Y, Abe M, Tabata T, Sakurai T
    • Organizer
      第92回日本薬理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] B-type GABA receptor serves as a dynamic modulator increasing the ligand-sensitivity of type-1 metabotropic glutamate receptor2018

    • Author(s)
      Abe M, Ikeda K, Ichiki A, Matsuoka K, Sakairi H, Kamikubo Y, Sakurai T, Tabata T
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Research-status Report
  • [Remarks] 富山大学工学部生体情報処理研究室ホームページ

    • URL

      http://www3.u-toyama.ac.jp/biophys/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 富山大学大学院理工学教育部生体情報処理研究室ホームページ

    • URL

      http://www3.u-toyama.ac.jp/biophys/

    • Related Report
      2019 Research-status Report
  • [Remarks] 富山大学工学部知能情報工学コース生体情報処理研究室ホームページ

    • URL

      http://www3.u-toyama.ac.jp/biophys/

    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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