• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A study on the participation of postsynaptic importin in synaptic plasticity.

Research Project

Project/Area Number 18K06467
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 46010:Neuroscience-general-related
Research InstitutionKitasato University

Principal Investigator

Itakura Makoto  北里大学, 医学部, 准教授 (30398581)

Co-Investigator(Kenkyū-buntansha) 小寺 義男  北里大学, 理学部, 教授 (60265733)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords核輸送因子 / インポーチン / 神経細胞 / 転写制御因子 / 細胞内情報伝達 / 核輸送 / タンパク質リン酸化 / 核内輸送 / シナプス伝達 / 定量プロテオーム解析 / シナプス可塑性 / プロテオーム解析 / シナプス / AMPA受容体 / TARP
Outline of Final Research Achievements

The trafficking of proteins between cytoplasm and nucleus through nuclear pore complexes is mediated by importin family. We aimed to clarify the role of importin β1 in neurons. The importin family is known to form heterodimers, and in the rodent brain, importin β1 was mainly bound to importins α3, α4, 7. Identification of transcription factors that bind to importin family in neurons revealed that they contained genes (TDP-43, MeCP2 and rogdi) involved in neurological disorders and neurogenesis. Nuclear transport by importins is regulated by small G protein Ran. It was revealed that RCC1 (RanGEF) and RanGAP1, which control the GDP-GTP exchange of Ran, are phosphorylated by nerve growth factor signaling pathway. This result suggests that nerve growth factors (NGF, BDNF) involved in various neural functions also control the nuclear transport of neurons.

Academic Significance and Societal Importance of the Research Achievements

高齢化社会では、加齢による認知症が増加しており、その治療法の開発が求められている。そのためには、記憶や学習の分子レベルでのメカニズム解明が非常に重要である。記憶や学習には、神経細胞間の神経伝達に依存した新規遺伝子の発現が必須であり、その際にはインポーチンファミリーを介した核内へのタンパク質輸送が起きていると考えられる。本研究の研究結果は、記憶や学習に関与する神経成長因子が、インポーチンを介する核輸送も制御している可能性を示している。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (4 results)

All 2021 2019

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results)

  • [Journal Article] A highly efficient method for extracting peptides from a single mouse hypothalamus2021

    • Author(s)
      Yuzuru Nakagawa, Takashi Matsui, Ryo Konno, Yusuke Kawashima, Toshiya Sato, Makoto Itakura, Yoshio Kodera
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 548 Pages: 155-160

    • DOI

      10.1016/j.bbrc.2021.02.041

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highly accurate and precise quantification strategy using stable isotope dimethyl labeling coupled with GeLC-MS/MS2021

    • Author(s)
      Ryo Konno, Takashi Matsui, Hiroaki Ito, Yusuke Kawashima, Makoto Itakura, Yoshio Kodera
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 550 Pages: 37-42

    • DOI

      10.1016/j.bbrc.2021.02.101

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 水中拘束ストレスマウスの大脳皮質を対象とした包括的なタンパク質存在様式の比較分析2019

    • Author(s)
      紺野 亮, 伊藤 大晃, 樋口 雅崇, 松井 崇, 佐藤 俊哉, 板倉 誠, 小寺 義男
    • Organizer
      日本プロテオーム学会、日本電気泳動学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 脳の機能状態モニタリングを目指した脳脊髄液の詳細な比較分析への取り組み2019

    • Author(s)
      角田 貴樹, 紺野 亮, 板倉 誠, 松井 崇, 小寺 義男
    • Organizer
      日本プロテオーム学会、日本電気泳動学会
    • Related Report
      2019 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi