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The elucidation of neurogenesis mechanism by novel RNA binding protein Marf1, related with 16p13.11 duplication syndrome

Research Project

Project/Area Number 18K06474
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 46010:Neuroscience-general-related
Research InstitutionShimane University

Principal Investigator

Fujitani Masashi  島根大学, 学術研究院医学・看護学系, 教授 (40376372)

Co-Investigator(Kenkyū-buntansha) 水野 誠司  愛知県医療療育総合センター発達障害研究所, 遺伝子医療研究部, 非常勤研究員 (20393150)
斎藤 潤  京都大学, iPS細胞研究所, 准教授 (90535486)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords神経新生 / sMarf1 / 16p13.11 / iPS 細胞 / Marf1 / iPS細胞 / 15q11-13 / 神経分化 / 発達障がい / 神経幹細胞
Outline of Final Research Achievements

In order to identify new target molecules of sMarf1 and to elucidate the molecular mechanisms involved in the neural differentiation of mouse and human neural stem cells, we attempted to identify mRNA of new target molecules using the RIP-Chip method. The RNAs that bind to sMarf1 were identified by microarray . Subsequently, we examined whether the overexpression of sMarf1 decreased the expression of the candidate molecules. However, there was no decrease in their expression. In addition, we identified several molecular targets by RNA-seq analysis, but since no decrease in expression was observed by real time PCR, we concluded that the identification of target molecules was extremely difficult.
We also generated human disease-specific iPS cells of 16p13.11 microduplication and analyze their neural differentiation potential. We evaluated the neural differentiation ability of the cells, but found no change.

Academic Significance and Societal Importance of the Research Achievements

本研究により、sMarf1の結合RNAを探索する実験は、失敗に終わった。RNA結合タンパク質のターゲット分子を同定する方法として、最近の大規模な研究によっても、Marf1のターゲットは未だ同定に至っていない。(nature 2020 Van Nostrand et al) この大規模な研究では、5つの大きな実験をおこなっているが、その中の可能な限りの2つの実験を行ったが、同定することはできなかった。また、16p13.11iPS細胞から分化させた神経細胞の神経分化能を評価したが、大きな変化はなかった。 今後は、神経分化能のみならず、神経細胞の形態に着目した研究を行っていきたいと考えている。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2021 2020 2019

All Journal Article (7 results) (of which Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (4 results) (of which Invited: 1 results)

  • [Journal Article] A peroxisome deficiency-induced reductive cytosol state up-regulates the brain-derived neurotrophic factor pathway2020

    • Author(s)
      Abe Yuichi、Honsho Masanori、Kawaguchi Ryoko、Matsuzaki Takashi、Ichiki Yayoi、Fujitani Masashi、Fujiwara Kazushirou、Hirokane Masaaki、Oku Masahide、Sakai Yasuyoshi、Yamashita Toshihide、Fujiki Yukio
    • Journal Title

      Journal of Biological Chemistry

      Volume: - Issue: 16 Pages: 5321-5334

    • DOI

      10.1074/jbc.ra119.011989

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 解剖献体を用いたCST(cadaver surgical training)について2020

    • Author(s)
      藤谷昌司
    • Journal Title

      西日本泌尿器科学会誌

      Volume: 82 Pages: 20-25

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 島根大学医学部におけるオンライン/オンデマンドの講義システムの構築2020

    • Author(s)
      藤谷昌司、山田壮史、永井誠大、天野 佑
    • Journal Title

      医学教育

      Volume: 51(3) Pages: 1-9

    • NAID

      130007873048

    • Related Report
      2020 Annual Research Report
  • [Journal Article] 島根大学医学部におけるCST(Cadaver Surgical Training)の現状と今後について2020

    • Author(s)
      藤谷昌司、平野了、渡部広明、大谷浩
    • Journal Title

      島根医学

      Volume: 40(1) Pages: 1-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 解剖献体を用いたCST(Cadaver Surgical Training)について2020

    • Author(s)
      藤谷昌司
    • Journal Title

      西日本泌尿器科学会誌

      Volume: 82 Pages: 20-25

    • Related Report
      2019 Research-status Report
  • [Journal Article] New nociceptive circuits to the hypothalamic perifornical area from the spinal cord and spinal trigeminal nucleus via the parabrachial nucleus2019

    • Author(s)
      Hirohiko Asano, Yosuke Arima, Shigefumi Yokota, Masashi Fujitani
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 512 Issue: 4 Pages: 705-711

    • DOI

      10.1016/j.bbrc.2019.02.153

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lateral parabrachial neurons innervate orexin neurons projecting to brainstem arousal areas in the rat2019

    • Author(s)
      Yosuke Arima, Shigefumi Yokota, Masashi Fujitani
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 2830-2830

    • DOI

      10.1038/s41598-019-39063-y

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 染色体15q11-13微小重複症ASDモデルマウスにおけるAISの長さの解析2021

    • Author(s)
      山中由芽、片瀬創平、大谷嘉典、藤谷昌司
    • Organizer
      第126回日本解剖学会総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 微小重複症モデル動物・細胞における神経生物学的解析2021

    • Author(s)
      藤谷昌司、大谷嘉典、山中由芽 、赤松和土
    • Organizer
      愛知県医療療育総合センター発達障害研究所セミナー
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 16p13.11 微小重複症の原因遺伝子解明を目指して2020

    • Author(s)
      藤谷昌司、有馬陽介、大谷嘉典、水上洋一
    • Organizer
      自閉症学研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 16p13.11 微小重複症の原因遺伝子解明を目指して2020

    • Author(s)
      藤谷昌司、有馬陽介、大谷嘉典、水上洋一
    • Organizer
      第10回自閉症学研究会
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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