Identification of the gene that defines a variety of the strength of plasticity in mouse primary visual cortex
| Project/Area Number |
18K06476
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 46010:Neuroscience-general-related
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
Hayashi Kenji 生理学研究所, 基盤神経科学研究領域, 助教 (50512349)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 一酸化窒素 / 眼優位可塑性 / 神経可塑性 / in vivo カルシウムイメージング / 遺伝子発現解析 / 一次視覚野 / RNAseq |
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| Outline of Final Research Achievements |
Nitric oxide (NO) is known to be a diffuse mediator for modifying the efficacy of synaptic transmission. In the cortex, NO relates to long-term plasticity by affecting neighboring cells, but the in vivo cortical function is elusive.
Here we show that cortical NO inhibits ocular dominance plasticity. We developed an AAV-DREADD based on Tet-system to specifically and sufficiently activate nitric oxide synthase 1 (NOS1) positive inhibitory cells in NOS1-IRES-Cre mice. We found that the chronic activation of NOS1+ cells eliminates the ocular dominance shift in monocularly deprived animals with NO-dependent manner. Diffusive NO can act synchronously on the plasticity of the surrounding cell population. Together, NO might play a crucial role as a pacemaker in orhestrate the development of the cell ensemble in visual cortex through reducing the instability.
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| Academic Significance and Societal Importance of the Research Achievements |
我々の研究によりin vivo大脳皮質でNOは可塑性に対して抑制的に働くことが分かってきた。眼優位可塑性の中でも制動の分子メカニズムはほぼ未解明であり、本研究の進展は眼優位可塑性の全体像の理解のための重要な知見となると考えている。
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Report
(4 results)
Research Products
(1 results)
