| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Nitric oxide (NO) is known to be a diffuse mediator for modifying the efficacy of synaptic transmission. In the cortex, NO relates to long-term plasticity by affecting neighboring cells, but the in vivo cortical function is elusive.
Here we show that cortical NO inhibits ocular dominance plasticity. We developed an AAV-DREADD based on Tet-system to specifically and sufficiently activate nitric oxide synthase 1 (NOS1) positive inhibitory cells in NOS1-IRES-Cre mice. We found that the chronic activation of NOS1+ cells eliminates the ocular dominance shift in monocularly deprived animals with NO-dependent manner. Diffusive NO can act synchronously on the plasticity of the surrounding cell population. Together, NO might play a crucial role as a pacemaker in orhestrate the development of the cell ensemble in visual cortex through reducing the instability.
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