Axonal regeneration through Photoactivated adenylyl cyclase

Research Project

Project/Area Number	18K06482
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 46010:Neuroscience-general-related
Research Institution	Hamamatsu University School of Medicine
Principal Investigator	Omura Takao 浜松医科大学, 医学部附属病院, 講師 (70402295)
Project Period (FY)	2018-04-01 – 2021-03-31
Project Status	Completed (Fiscal Year 2020)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	神経再生 / 中枢神経損傷 / PAC / 視神経損傷 / 中枢神経再生 / 光活性化アデニル酸シクラーゼ / アデノ随伴ウイルス / マウス後根神経節 / マウス視神経 / cAMP
Outline of Final Research Achievements	We overexpressed Photoactivated adenylyl cyclase(PAC) in the DRG sensory neurons　through AAV and analyzed axonal growth and neuronal sprouting in comparison with the control AAV. WE found that activation of PAC significantly increased axonal growth and sprouting in the DRG neurons. We next used in vivo optic nerve injury model to see whether PAC was able to promote axonal growth in the CNS. WE discovered that activation of PAC though AAV induced axonal growth after optic nerve injury, when no growth was observed in the control virus group/
Academic Significance and Societal Importance of the Research Achievements	脳梗塞、脊髄損傷などの中枢神経損傷に対する有効な治療法は確立されておらず、これら疾患から回復することは極めて困難である。我々の研究ではPACを介し、中枢神経の損傷モデルである視神経損傷において軸索再生の促進が見られたことにより、今後中枢神経損傷患者に対する治療の一助となる可能性が示唆された。