| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
This project focused on the molecular mechanism of synaptic stabilization. First, dendritic spines of cortical pyramidal neurons were imaged in vivo using two-photon microscopy over time to identify stably maintained spines and newly emerging spines. The same dendrites were then examined by electron microscopy to obtain ultrastructural images of dendritic spines by three-dimensional reconstruction. The results showed that the neck length of the stable spines was almost the same as that of the newly emerged spines, but the spine head of the stable spines was larger and the morphology more complex. In addition, to elucidate the molecular mechanism of synaptic stabilization, we clarified the role of teneurin, a membrane protein involved in synaptic induction.
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