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Suppression of protein aggregate formation in ALS and other neurodegenerative disease models

Research Project

Project/Area Number 18K06507
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 46020:Anatomy and histopathology of nervous system-related
Research InstitutionKyorin University

Principal Investigator

Watabe Kazuhiko  杏林大学, 保健学部, 教授 (30240477)

Co-Investigator(Kenkyū-buntansha) 村田 麻喜子  杏林大学, 保健学部, 講師 (00276205)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords筋萎縮性側索硬化症 / アデノウイルス / TDP-43 / Praja1 / FUS / SOD1 / α-synuclein / polyglutamine / 運動ニューロン / 蛋白質凝集体 / 組換えウイルス / プロテアソーム / HSF1 / 筋萎縮性側索硬化症 (ALS) / Praja1 (PJA1) / 熱ショック応答 / 凝集体 / Praja 1 (PJA1) / ALS / 組換えアデノウイルス
Outline of Final Research Achievements

The formation of misfolded protein aggregates is one of the pathological hallmarks of neurodegenerative diseases. We demonstrated that, in neuronal cell culture and mouse models, Praja1 RING-finger E3 ubiquitin ligase (PJA1) suppresses the cytoplasmic aggregate formation of transactivation response DNA-binding protein of 43kDa (TDP-43), the main constituent of neuronal cytoplasmic aggregates in cases of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Furthermore, PJA1 also suppressed the aggregate formation of fused in sarcoma, superoxide dismutase 1, α-synuclein, and ataxin-3 and huntingtin polyglutamine proteins in neuronal cultures. These results suggest that PJA1 is a common sensing factor for aggregate-prone proteins to counteract their aggregation propensity and could be a potential therapeutic target for neurodegenerative diseases that include ALS, FTLD, Parkinson’s disease, and polyglutamine diseases.

Academic Significance and Societal Importance of the Research Achievements

筋萎縮性側索硬化症,パーキンソン病,ポリグルタミン病などの神経変性疾患では,神経細胞内に各々TDP-43, FUS, SOD1, α-synuclein, ataxin 3, huntingtinなどの疾患特異的蛋白質からなる不溶性凝集体が形成され,凝集体の細胞間伝播とともに神経細胞変性が進行する.本研究ではPraja1 E3ユビキチンリガーゼ (PJA1) がこれら蛋白質の凝集を抑制することを見出し,PJA1による蛋白凝集抑制メカニズムの解明・応用が神経変性疾患全般の治療法開発に寄与する可能性が示唆された.

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (28 results)

All 2021 2020 2019 2018

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (20 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] 組換えウイルスを用いた筋萎縮性側索硬化症病変の発症進展機序の解明.2021

    • Author(s)
      渡部和彦
    • Journal Title

      細胞

      Volume: 53 Pages: 50-53

    • NAID

      40022520041

    • Related Report
      2020 Research-status Report
  • [Journal Article] Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.2020

    • Author(s)
      Watabe K, Kato Y, Sakuma M, Murata M, Niida-Kawaguchi M, Takemura T, Hanagata N, Tada M, Kakita A, Shibata N.
    • Journal Title

      Neuropathology

      Volume: 40 Issue: 6 Pages: 570-586

    • DOI

      10.1111/neup.12694

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A low amyloidogenic E61K transthyretin mutation may cause familial amyloid polyneuropathy.2020

    • Author(s)
      Murakami T, Yokoyama T, Mizuguchi M, Toné S, Takaku S, Sango K, Nishimura H, Watabe K, Sunada Y.
    • Journal Title

      J Neurochem

      Volume: 156 Issue: 6 Pages: 957-966

    • DOI

      10.1111/jnc.15162

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Astrocytes release glutamate via cystine/glutamate antiporter upregulated in response to increased oxidative stress related to sporadic amyotrophic lateral sclerosis.2020

    • Author(s)
      Kazama M, Kato Y, Kakita A, Noguchi N, Urano Y, Masui K, Niida-Kawaguchi M, Yamamoto T, Watabe K, Kitagawa K, Shibata N.
    • Journal Title

      Neuropathology

      Volume: 40 Issue: 6 Pages: 587-598

    • DOI

      10.1111/neup.12716

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Soluble iron accumulation induces microglial glutamate release in the spinal cord of sporadic amyotrophic lateral sclerosis.2020

    • Author(s)
      Niida-Kawaguchi M, Kakita A, Noguchi N, Kazama M, Masui K, Kato Y, Yamamoto T, Sawada T, Kitagawa K, Watabe K, Shibata N.
    • Journal Title

      Neuropathology

      Volume: 40 Issue: 2 Pages: 152-166

    • DOI

      10.1111/neup.12632

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] 組換えウイルスを用いた筋萎縮性側索硬化症病変の発症進展機序の解明.2019

    • Author(s)
      渡部和彦
    • Journal Title

      Medical Science Digest

      Volume: 45 Pages: 191-194

    • NAID

      40022520041

    • Related Report
      2018 Research-status Report
  • [Journal Article] A spontaneously immortalized Schwann cell line from aldose reductase-deficient mice as a useful tool for studying polyol pathway and aldehyde metabolism.2018

    • Author(s)
      Niimi N, Yako H, Takaku S, Kato H, Matsumoto T, Nishito Y, Watabe K, Ogasawara S, Mizukami H, Yagihashi S, Chung SK, Sango K.
    • Journal Title

      J Neurochem

      Volume: 144 Issue: 6 Pages: 710-722

    • DOI

      10.1111/jnc.14277

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] SIMPLE binds specifically to PI4P through SIMPLE-like domain and participates in protein trafficking in the trans-Golgi network and/or recycling endosomes.2018

    • Author(s)
      Moriwaki Y, Ohno Y, Ishii T, Takamura Y, Kita Y, Watabe K, Sango K, Misawa H.
    • Journal Title

      PLoS One

      Volume: 13 Issue: 6 Pages: e0199829-e0199829

    • DOI

      10.1371/journal.pone.0199829

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Praja1, ZNF179ユビキチンリガーゼのTDP-43凝集体形成抑制効果.2021

    • Author(s)
      渡部和彦,加藤陽一郎,村田麻喜子,新井田素子,他田真理,柿田明美,柴田亮行.
    • Organizer
      第62回日本神経学会学術大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Praja1, ZNF179ユビキチンリガーゼのTDP-43凝集体形成抑制効果.2021

    • Author(s)
      渡部和彦,加藤陽一郎,村田麻喜子,新井田素子,他田真理,柿田明美,柴田亮行.
    • Organizer
      第62回日本神経病理学会総会学術研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ミクログリアにおける家族性ALS変異SOD1蛋白分解機構の解明.2021

    • Author(s)
      新井田素子,須藤則宏,塚原富士子,渡部和彦,柴田亮行.
    • Organizer
      第62回日本神経病理学会総会学術研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Praja1, ZNF179 E3ビキチンリガーゼのTDP-43凝集体形成抑制効果.2021

    • Author(s)
      渡部和彦,加藤陽一郎,村田麻喜子,新井田素子,他田真理,柿田明美,柴田亮行.
    • Organizer
      第43回日本神経科学大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 培養神経変性疾患モデルにおける蛋白質凝集とその抑制.2021

    • Author(s)
      渡部和彦.
    • Organizer
      第43回神経組織培養研究会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Praja 1 E3 ubiquitin ligase suppresses adenovirus-induced neuronal TDP-43 aggregate formation.2020

    • Author(s)
      渡部和彦,加藤陽一郎,佐久間美帆,村田麻喜子,新井田素子,竹村太郎,花方信孝,柿田明美,柴田亮行.
    • Organizer
      第61回日本神経学会学術大会
    • Related Report
      2020 Research-status Report
  • [Presentation] TTR E61K アミロイドニューロパチーの神経変性機序の研究..2020

    • Author(s)
      村上龍文, 刀祢重信, 三五一憲, 水口峰之, 渡部和彦, 砂田 芳秀.
    • Organizer
      第61回日本神経学会学術大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Praja 1 RING-finger E3 ubiquitin ligaseによるTDP-43細胞質凝集体の形成抑制.2020

    • Author(s)
      渡部和彦,加藤陽一郎,佐久間美帆,村田麻喜子,新井田素子,竹村太郎,花方信孝,柿田明美,柴田亮行.
    • Organizer
      第63回日本神経化学大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Praja 1 E3 ubiquitin ligaseによるTDP-43細胞質凝集体の形成抑制.2020

    • Author(s)
      渡部和彦,加藤陽一郎,佐久間美帆,村田麻喜子,新井田素子,竹村太郎,花方信孝,柿田明美,柴田亮行.
    • Organizer
      第61回日本神経病理学会総会学術研究会
    • Related Report
      2020 Research-status Report
  • [Presentation] ALS細胞質TDP-43凝集体形成抑制分子の同定と機能解析.2020

    • Author(s)
      渡部和彦.
    • Organizer
      第42回神経組織培養研究会
    • Related Report
      2020 Research-status Report
  • [Presentation] 筋萎縮性側索硬化症 (ALS) におけるアストロサイト増殖性の検討.2019

    • Author(s)
      新井田(川口)素子,渡部和彦,山本智子,加藤陽一郎,柴田亮行.
    • Organizer
      第108回日本病理学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] 培養ニューロン細胞質TDP-43凝集体形成を抑制する分子の探索.2019

    • Author(s)
      渡部和彦,加藤陽一郎,佐久間美帆,村田麻喜子,新井田(川口)素子,柿田明美,柴田亮行.
    • Organizer
      第60回日本神経病理学会総会学術研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] 培養ニューロン細胞質TDP-43凝集体形成を抑制する分子の解析.2019

    • Author(s)
      渡部和彦,加藤陽一郎,佐久間美帆,村田麻喜子,新井田(川口)素子,柿田明美,柴田亮行.
    • Organizer
      第42回日本神経科学大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 培養ニューロン細胞質TDP-43凝集体形成を抑制する分子の解析.2019

    • Author(s)
      渡部和彦,加藤陽一郎,佐久間美帆,村田麻喜子,新井田(川口)素子,竹村太郎,花方信孝,柿田明美,柴田亮行.
    • Organizer
      第14回日本臨床ストレス応答学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Characterization of astrocytes associated with amyotrophic lateral sclerosis (ALS).2019

    • Author(s)
      新井田素子,渡部和彦,山本智子,柴田亮行.
    • Organizer
      第41回神経組織培養研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] 熱ショック応答関連分子によるTDP-43細胞質凝集体形成の抑制効果.2018

    • Author(s)
      渡部和彦,加藤陽一郎,佐久間美帆,村田麻喜子,新井田素子,柿田明美,柴田亮行.
    • Organizer
      第59回日本神経学会学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 熱ショック応答によるTDP-43細胞質凝集体の形成抑制効果.2018

    • Author(s)
      渡部和彦,加藤陽一郎,佐久間美帆,村田麻喜子,新井田素子,柿田明美,柴田亮行.
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Excessive soluble iron stimulates microglia to release glutamate in ALS spinal cords.2018

    • Author(s)
      Shibata N, Niida-Kawaguchi M, Masui K, Kakita A, Watabe K.
    • Organizer
      19th International Congress of Neuropathology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] HSF1 suppresses adenovirus-induced neuronal TDP-43 aggregate formation in culture.2018

    • Author(s)
      Watabe K, Kato Y, Sakuma M, Murata M, Niida-Kawaguchi M, Kakita A, Shibata N.
    • Organizer
      19th International Congress of Neuropathology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 家族性アミロイドポリニューロパチーTTR E61Kの神経変性機序の研究:アミロイド凝集能の検討.2018

    • Author(s)
      村上龍文,刀祢重信,三五一憲,渡部和彦,水口峰之,砂田芳秀.
    • Organizer
      第29回日本末梢神経学会学術集会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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