• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of partially fluorinated lipid/membrane protein complex designed based on Stratified Dipole-Arrays model

Research Project

Project/Area Number 18K06568
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 47010:Pharmaceutical chemistry and drug development sciences-related
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Takagi Toshiyuki  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (10248065)

Co-Investigator(Kenkyū-buntansha) 園山 正史  群馬大学, 大学院理工学府, 教授 (40242242)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsペルフルオロアルキル基 / 人工脂質 / 膜タンパク質 / 再構成膜 / バクテリオロドプシン / 多重積層アレー双極子モデル / DPPC / DMPC / ペルフルオロアルキル / バイオセンサ / フッ素 / SDAモデル
Outline of Final Research Achievements

Sufficient long perfluoroalkyl chains are strongly hydrophobic and more rigid than hydrocarbon ones, leading to the formation of rigid assemblies, of which properties are not suitable for membrane protein reconstitution matrices. Partially fluorinated phospholipid-based membranes, however, are greatly expected promising materials for reconstitution of membrane proteins.
In this study, we presented synthesis of phospholipids (DMPC and DPPC) having perfluoroalkyl groups of different lengths and their membrane properties. Our proposed fluorinated phospholipids designed based on Stratified Dipole-Arrays (SDA) model are considered effective to provide the mechanically stable and fluid membranes, essential for the construction of membrane protein reconstitution matrices.

Academic Significance and Societal Importance of the Research Achievements

膜タンパク質を標的とした医薬品開発において、脂質・膜タンパク質複合体膜は、医薬品スクリーニングの現場に有望である。そこで、脂質・膜タンパク質複合体膜における膜タンパク質の機能を長期安定維持可能な人工脂質の開発として、フッ素導入した擬環状型脂質の開発を目指している。SDA理論に基づく含フッ素化界面活性剤設計は、人工血管材料の表面修飾剤やリポソームDDS材料や臨床診断細胞分散剤などの医療やバイオ分野領域における研究開発を加速すると期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (26 results)

All 2021 2020 2019 2018

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (13 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Membrane properties of amacrocyclic tetraether bisphosphatidylcholine lipid: Effect of a single membrane-spanning polymethylene cross-linkage between two head groups of ditetradecylphosphatidylcholine membrane2021

    • Author(s)
      Tsuchida Naoyuki、Takagi Toshiyuki、Takahashi Hiroshi、Yoshihara Toshitada、Tobita Seiji、Sonoyama Masashi
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Biomembranes

      Volume: 1863 Issue: 5 Pages: 183569-183569

    • DOI

      10.1016/j.bbamem.2021.183569

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Membrane properties of ether-type phosphatidylcholine bearing partially fluorinated C18-monoacetylenic chains and their applicability to membrane protein reconstitution matrices2021

    • Author(s)
      Baba Teruhiko、Takagi Toshiyuki、Sumaru Kimio、Kanamori Toshiyuki、Dewa Takehisa、Nango Mamoru
    • Journal Title

      Colloids and Surfaces B: Biointerfaces

      Volume: 198 Pages: 111459-111459

    • DOI

      10.1016/j.colsurfb.2020.111459

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of salt and gel network structures on purple membrane stacking in hydrogels immobilized with poly(vinyl alcohol)2021

    • Author(s)
      Yokoyama Yasunori、Yano Shunsuke、Kurita Riku、Karasawa Morise、Tanaka Hikaru、Takahashi Hiroshi、Kikukawa Takashi、Sonoyama Masashi、Takenaka Koshi
    • Journal Title

      Journal of Applied Physics

      Volume: 129 Issue: 1 Pages: 014701-014701

    • DOI

      10.1063/5.0032546

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of the fluorination degree of partially fluorinated octyl-phosphocholine surfactants on their interfacial properties and interactions with purple membrane as a membrane protein model2020

    • Author(s)
      Baba Teruhiko、Takagi Toshiyuki、Sumaru Kimio、Kanamori Toshiyuki
    • Journal Title

      Chemistry and Physics of Lipids

      Volume: 227 Pages: 104870-104870

    • DOI

      10.1016/j.chemphyslip.2020.104870

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Thermotropic Transition Behaviors of Novel Partially Fluorinated Dimyristoylphosphatidylcholines with Different Perfluoroalkyl Chain Lengths2019

    • Author(s)
      Morita Kohei、Horikoshi Miki、Yanagi Tamami、Takagi Toshiyuki、Takahashi Hiroshi、Amii Hideki、Hasegawa Takeshi、Sonoyama Masashi
    • Journal Title

      Chemistry Letters

      Volume: 48 Issue: 9 Pages: 1105-1108

    • DOI

      10.1246/cl.190389

    • NAID

      130007703026

    • ISSN
      0366-7022, 1348-0715
    • Year and Date
      2019-09-05
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison of two different partially fluorinated phosphatidylcholines with the perfluorobutyl group on thermotropic properties of the bilayer membrane and reconstituted bacteriorhodopsin2019

    • Author(s)
      Yanagi Tamami、Takagi Toshiyuki、Takahashi Hiroshi、Kikukawa Takashi、Amii Hideki、Sonoyama Masashi
    • Journal Title

      Biophysical Reviews

      Volume: 11 Issue: 3 Pages: 395-398

    • DOI

      10.1007/s12551-019-00540-3

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aggregation behavior of short-chained archaeal phospholipid analogs: Contribution of methyl branches to lipid hydrophobicity and membrane formability2019

    • Author(s)
      Komatsu Hiroshi、Sakai Kiyotaka、Baba Teruhiko、Takagi Toshiyuki、Kanamori Toshiyuki
    • Journal Title

      Colloid and Interface Science Communications

      Volume: 32 Pages: 100200-100200

    • DOI

      10.1016/j.colcom.2019.100200

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Partially Fluorinated Phospholipid Bilayer2019

    • Author(s)
      SONOYAMA Masashi、TAKAGI Toshiyuki、TAKAHASHI Hiroshi
    • Journal Title

      Seibutsu Butsuri

      Volume: 59 Issue: 6 Pages: 310-313

    • DOI

      10.2142/biophys.59.310

    • NAID

      130007751044

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2019 Research-status Report
  • [Journal Article] Fluorous Property of a Short Perfluoroalkyl-Containing Compound Realized by Self-Assembled Monolayer Technique on a Silicon Substrate2019

    • Author(s)
      Kise Ryuma、Fukumi Aki、Shioya Nobutaka、Shimoaka Takafumi、Sonoyama Masashi、Amii Hideki、Takagi Toshiyuki、Kanamori Toshiyuki、Eda Kazuo、Hasegawa Takeshi
    • Journal Title

      Bulletin of the Chemical Society of Japan

      Volume: 92 Issue: 4 Pages: 785-789

    • DOI

      10.1246/bcsj.20180356

    • NAID

      130007632600

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Raman Optical Activity on a Solid Sample: Identification of Atropisomers of Perfluoroalkyl Chains Having a Helical Conformation and No Chiral Center2019

    • Author(s)
      Shimoaka Takafumi、Sonoyama Masashi、Amii Hideki、Takagi Toshiyuki、Kanamori Toshiyuki、Hasegawa Takeshi
    • Journal Title

      The Journal of Physical Chemistry A

      Volume: 123 Issue: 18 Pages: 3985-3991

    • DOI

      10.1021/acs.jpca.8b11613

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Stability of the two-dimensional lattice of bacteriorhodopsin reconstituted in partially fluorinated phosphatidylcholine bilayers2019

    • Author(s)
      Takahashi Hiroshi、Yoshino Masaru、Morita Kohei、Takagi Toshiyuki、Yokoyama Yasunori、Kikukawa Takashi、Amii Hideki、Kanamori Toshiyuki、Sonoyama Masashi
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Biomembranes

      Volume: 1861 Issue: 3 Pages: 631-642

    • DOI

      10.1016/j.bbamem.2018.12.015

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synthesis, Membrane Structure and Physical Properties of Partially Fluorinated Phospholipids, and Prospects of Their Use for Membrane Protein Research2019

    • Author(s)
      Takahashi Hiroshi、Takagi Toshiyuki、Sonoyama Masashi
    • Journal Title

      MEMBRANE

      Volume: 44 Issue: 2 Pages: 50-56

    • DOI

      10.5360/membrane.44.50

    • NAID

      130007623271

    • ISSN
      0385-1036, 1884-6440
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Nanodiscに単離したバクテリオロドプシン三量体の光サイクルと安定性2021

    • Author(s)
      黒澤紋音・徳留俊・林史夫・菊川峰志・白石充典・園山正史
    • Organizer
      日本生物物理学会関東支部会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Partially Fluorinated Phospholipid Membrane2020

    • Author(s)
      1.Masashi Sonoyama
    • Organizer
      58th Annual Meeting of Biophysical Society of Japan
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ヒト脳細胞由来脂肪酸結合タンパク質 FABP の脂質二重膜を介した脂肪酸結合親和性解析2020

    • Author(s)
      徳留 俊・野村 舞・林 史夫・杉山 成・松岡 茂・村田 道雄・園山 正史
    • Organizer
      日本化学会関東支部群馬地区研究交流発表会
    • Related Report
      2020 Annual Research Report
  • [Presentation] フッ素化したエーテル型脂質dihexadecylphosphatydilcholineの合成と熱物性の解析2020

    • Author(s)
      笹木菜々子、高木俊之、高橋浩、園山正史
    • Organizer
      第9回日本生物物理学会関東支部会
    • Related Report
      2019 Research-status Report
  • [Presentation] Raman optical activity specifically found on solid samples of perfluoroalkyl compounds having helical conformation and no chiral center2019

    • Author(s)
      T. Shimoaka, M. Sonoyama, H. Amii, T. Takagi, T. Kanamori, T. Hasegawa
    • Organizer
      International Conference on Advanced Vibrational Spectroscopy
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 擬環状エーテル型脂質のフッ素化が膜物性および膜タンパク質の構造・機能に及ぼす影響2019

    • Author(s)
      川原るい、高木俊之、高橋浩、菊川峰志、網井秀樹、園山正史
    • Organizer
      第42回フッ素化学討論会
    • Related Report
      2019 Research-status Report
  • [Presentation] パーフルオロアルキル鎖長に依存した部分フッ素化リン脂質二分子膜の相転移挙動2019

    • Author(s)
      栁瑶美、高木俊之、高橋浩、網井秀樹、長谷川健、金森敏幸、園山正史
    • Organizer
      第18回産総研・産技連LS-BT合同研究発表会
    • Related Report
      2019 Research-status Report
  • [Presentation] Thermal Stability of the Hexagonal Lattice Packing Structure of Bacteriorhodopsin in Partially Fluorinated Phospholipid Bilayers 59th International Conference on the Bioscience of Lipids2018

    • Author(s)
      M. Yoshino, H. Takahashi, Y. Yokoyama, T. Takagi, H. Amii, T. Kanamori, M. Kikukawa, M. Sonoyama
    • Organizer
      59TH INTERNATIONAL CONFERENCE ON THE BIOSCIENCE OF LIPIDS
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] パーフルオロアルキル鎖特有のねじれ構造に起因するアトロプ異性体のラマン分光イメージング法による識別2018

    • Author(s)
      下赤卓史、園山正史、網井秀樹、高木俊之、金森敏幸、長谷川健
    • Organizer
      日本分析化学会第67年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 部分フッ素化リン脂質二分子膜の熱物性2018

    • Author(s)
      森田康平、栁珠美、堀越未希、高木俊之、高橋浩、網井秀樹、茂木俊憲、金森敏幸、長谷川健、園山正史
    • Organizer
      第41回フッ素化学討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] パーフルオロアルキル鎖のねじれ構造に起因するアトロプ異性のラマン分光法による識別2018

    • Author(s)
      下赤卓史、園山正史、網井秀樹、高木俊之、金森敏幸、長谷川健
    • Organizer
      第41回フッ素化学討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] Thermotropic Phase Transition Behavior and Structural Properties of Partially Fluorinated Dipalmitoylphosphatidylcholine Bilayer2018

    • Author(s)
      T. Yanagi, H. Takahashi, T. Takagi, H. Amii, T. Motegi, T. Hasegawa, T. Kanamori, M. Sonoyama
    • Organizer
      Asian Biophysics Association Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Structure and Thermotropic Phase Transition Behavior of Perfluoroalkyl-Containing Dipalmitoylphosphatidylcholine Bilayer2018

    • Author(s)
      T. Yanagi, H. Takahashi, T. Takagi, H. Amii, T. Motegi, T. Hasegawa, T. Kanamori, M. Sonoyama
    • Organizer
      The 6th International Symposium of Gunma University Initiative for Advanced Research
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Book] 膜タンパク質工学ハンドブック2020

    • Author(s)
      秋吉一成、園山正史、富田泰輔
    • Total Pages
      624
    • Publisher
      (株)エヌ・ティー・エス
    • Related Report
      2019 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi