| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this research, we investigated mechanisms of an enzymatic reaction and inhibitor binding of an oxidized nucleotide hydrolase. We carried out the crystallization under microgravity and determined the high-resolution crystal structure. The structure revealed the protonation state in the active site with higher accuracy and precision. In addition, we obtained structural insights into the substrate specificity of the hydrolase by experiments of X-ray crystallography, activity measurement, and substrate binding.
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