Research Project
Grant-in-Aid for Scientific Research (C)
We focused on proton-pumping ATPase as a target for novel anti-bacterial agents since the enzymes are essential for bacterial growth and survival and are composed of various subunits with many pharmacophores.We studied the effects of inhibitors of proton-pumping F-ATPase on the growth and survival of oral pathogenic bacteria, S. mutans and S. anginosus under acidic conditions. F-ATPase inhibitors decreased the growth of these bacteria pH5.3. They also significantly reduced the colony-forming ability of them after incubation at pH 4.3, while showing less effect at pH 7.3. These observations indicate that S. mutans and S. anginosus are highly sensitive to F-ATPase inhibitors under acidic conditions. F-ATPase deficient S. anginosus exhibited essentially the same phenotype as that of wild-type with F-ATPase inhibitors, suggesting that F-ATPase plays an important role in acid tolerance of these bacteria.
本研究課題の成果により、口腔内病原細菌であるS. mutans、S. anginosusの耐酸性にプロトン輸送ATPaseが重要であることが示唆された。また、複数のポリフェノール化合物が同酵素を阻害し、これらの細菌の耐酸性を低下させることを明らかにした。プロトン輸送ATPaseは多数のサブユニットから構成される巨大分子であり、作用点の異なる阻害化合物を組み合わせることでよりより強力な抗菌作用が期待できる。また、ポリフェノール類は食品にも含まれるため、安全性が高い。本研究成果は、新たなう蝕・歯周病の予防薬、または口腔ケア用品の開発につながると考えられる
All 2021 2020 2019 2018
All Journal Article (4 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 4 results, Open Access: 3 results) Presentation (14 results) (of which Int'l Joint Research: 2 results) Patent(Industrial Property Rights) (1 results)
The Journal of Biochemistry
Volume: - Issue: 4 Pages: 459-466
10.1093/jb/mvaa118
Scientific Reports
Volume: 9 Issue: 1 Pages: 13587-13587
10.1038/s41598-019-49984-3
Archives of Biochemistry and Biophysics
Volume: 666 Pages: 46-51
10.1016/j.abb.2019.03.014
Volume: 8 Issue: 1 Pages: 6701-6701
10.1038/s41598-018-24918-7
