| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Mucin MUC5AC was identified as a possible Gal-2 ligand in mouse gastric mucus fraction by affinity chromatography and LC/MS/MS and it was revealed that Gal-2 could interact with MUC5AC in a β-galactoside-dependent manner. In the presence of lactose, Gal-2 retained its activity under oxidative environment. The results indicated that the presence of a carbohydrate ligand as well as prevention of oxidation by S-nitrosylation could contribute towards maintaining Gal-2 activity under oxidative environment in the gastric mucosa. By immunohistochemical study, galectin-2 immunoreaction was mainly found in cytosol of gastric surface mucous cells while MUC5AC immunoreaction was found in mucosal barrier as well as in mucous granules of mucous cells. Lectin histochemical analysis using Gal-2-GFP showed glycans recognized by Gal-2 were expressed in mucous granules of mucous cells. The results suggested that Gal-2 might play a role in the secretion of MUC5AC from surface mucous cells.
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