| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The characteristics of bacteria take up mercury into cells via Mer superfamily, i.e., MerC, MerE, MerF, MerT, or MerP, have been applied in engineering of bioreactor used for phytoremediation. Our objective was to clarify individual role of MerC, MerE, MerF, MerT, or MerP and potential in transport of toxic metals and rare metals. The E. coli cells that carried mer recombinants were treated with Cu, Au, or Sb. MerC or MerT+MerP recombinants accumulated significantly more Cu and Au than control. The cells that expressed MerC, MerE, MerF, or MerT accumulated significantly more Sb than control. Consequently, we demonstrated that MerC, MerE, MerF, and MerT are broad-spectrum metals transporters. Our results suggested that MerC is the most efficient tool and it was expected that not only the purification of toxic metal pollution but also the recovery of rare metals could be achieved.
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