| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
The aim of this study is elucidation of the biological roles of EXTL3, a biosynthetic enzyme of heparan sulfate. Brain glutamatergic nerve-specific Extl3-deficient mice were successfully generated and their behavioral as well as physical phenotypes were investigated. So far, typical abnormalities have not been observed. It may be involved in the higher functions in brain. Other enzymes may compensate the enzymatic activity of Extl3. T cell-specific Extl3-deficient mice were also successfully generated. Using the spleenish cells, differentiation of T cells was examined, and it turned out to tend to be immature. Cells of a patient with mutation in EXTL3 were cultured and the amount as well as the disaccharide compositions of glycosaminoglycans from them were examined. Heparan sulfate from the patient was reduced compared with that from a healthy subject.
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