Mechanistic study of anti-inflammatory activity of flavonoid mixtures and its application
| Project/Area Number |
18K06734
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 47050:Environmental and natural pharmaceutical resources-related
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| Research Institution | Keio University |
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Principal Investigator |
Kiuchi Fumiyuki 慶應義塾大学, 薬学部(芝共立), 教授 (60161402)
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| Co-Investigator(Kenkyū-buntansha) |
植草 義徳 慶應義塾大学, 薬学部(芝共立), 助教 (30753024)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | フラボノイド / 抗炎症作用 / 一酸化窒素(NO)産生抑制 / プロスタグランジン(PG)産生抑制 / オウゴン / NO産生抑制 / PG産生 / ポリメトキシフラボン / リン脂質膜親和性 / 混合物 / 一酸化窒素 / PGE2 |
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| Outline of Final Research Achievements |
Anti-inflammatory effect of an equimolar mixture of four flavonoids, baicalein (1), wogonin (2), oroxylin A (3), 6-methoxywogonin (4) contained in Scutellaria root, was analyzed using inhibition of the lipopolysaccharide-induced production of prostaglandin E2 (PGE2) and nitric oxide (NO) in cultured cells. A mixture of 1～3 (3-mix) inhibited the production of PGE2 stronger (synergistic effect) than that of a sum of each compound and addition of 4 to the 3-mix did not enhance the activity. On the contrary, the 3-mix did not show synergism in the inhibition of NO production, but addition of 4 exserted synergistic effect.
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| Academic Significance and Societal Importance of the Research Achievements |
生薬に含まれる構造類似の複数の成分を混合することにより，各成分単独よりも強い抗炎症作用を持つことを示した本研究の成果は，漢方処方に代表される複合医薬品の有効性の裏付けとなるとともに，少量の成分を組み合わせて各成分単独よりも強い効果を引き出す新たな医薬品の開発の可能性を示したものである．この研究を発展させることにより，複数の生薬成分を組合せることによって、漢方処方の特定の薬効を化合物として規格化できる医薬品で再現できるものと期待される．
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Report
(4 results)
Research Products
(3 results)
