| Project/Area Number |
18K06737
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 47050:Environmental and natural pharmaceutical resources-related
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
井戸 章子 岐阜薬科大学, 薬学部, 助教 (00336629)
中西 剛 岐阜薬科大学, 薬学部, 教授 (50303988)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 核内受容体 / フジツボ / RXR / EcR / レポーターアッセイ / 有機スズ化合物 / リガンド結合実験 / RACE法 |
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| Outline of Final Research Achievements |
We cloned a protein coding sequence of retinoid X receptor (RXR) and a partial sequence of ecdysone receptor from Fistulobalanus albicostatus (EcR), a species of barnacle. In order to extend our knowledge of the evolution of RXR and EcR in balanidae, we performed a phylogenetic analysis for amino acid sequences of RXR and EcR using the maximum likelihood method. The results suggest that the charactarization of barnacle EcR resembles that of arthropod EcRs but not barnacle RXR.
To investigate the binding affinity of barnacle RXR to 9-cis retinoic acid (9cRA) and triphenyltin (TPT), we performed the ligand binding assay. Although TPT bound to barnacle RXR, 9cRA failed to bind to to barnacle RXR.
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| Academic Significance and Societal Importance of the Research Achievements |
無脊椎動物における核内受容体に関する研究は、脊椎動物に比べると遅れているのが現状である。本研究では、無脊椎動物であるフジツボよりEcRおよびRXRを新たにクローニングする事に成功した。この成果は、無脊椎動物における核内受容体に関する知見を広げるものであると共に、無脊椎動物の生態系に及ぼす環境化学物質の影響を推定する足がかりになることが期待される。
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