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Functional analysis of streptococcal toxins

Research Project

Project/Area Number 18K06764
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 47060:Clinical pharmacy-related
Research InstitutionSuzuka University of Medical Science

Principal Investigator

OHKURA Kazuto  鈴鹿医療科学大学, 薬学部, 教授 (00242850)

Co-Investigator(Kenkyū-buntansha) 田端 厚之  徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 講師 (10432767)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords細菌感染症 / 細胞溶解毒素 / 生体膜 / cytolysin / 感染症
Outline of Final Research Achievements

・Streptococcus anginosus subsp. anginosus (SAA) was a hemolytic strain that produced the peptide hemolytic factor streptolysin S (SLS) and exhibited β-hemolysis. It was revealed that SLS functions as a cytotoxic factor.
・Mitis group Streptococcus pseudopneumoniae (SPpn) produced mitilectin (MLC). MLC had a lipase domain, two lectin domains, and a CDC partial domain. The MLC recombinant showed lipase activity and human-derived cell binding, but did not show hemolytic property and functioned as an adhesion molecule to human cells.

Academic Significance and Societal Importance of the Research Achievements

これら連鎖球菌関連の病原因子について、動的構造と生物活性の相関を考慮して作用機構の解析を進めている例は、国内外において申請者のグループ以外にはなく、溶血、血栓形成、血管炎といった細菌感染に起因する様々な症例の発症および重篤化のメカニズムの解明と、その予防・治療法の確立に繋がると思われる。さらに本研究は、連鎖球菌のみならず、様々な病原微生物と宿主の相互作用の進化論的研究や創薬、新医療技術の開発にも繋がると考えられ、その学問的、社会的意義は大きいといえる。加えて、食事内容(糖類)により細胞障害毒素の産生分泌や活性発現が制御できれば、潰瘍性大腸炎などの発症予防、治療方法の開発に繋がると期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (12 results)

All 2021 2020 2019 2018

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (6 results)

  • [Journal Article] Molecular characteristics of an adhesion molecule containing cholesterol‐dependent cytolysin‐motif produced by mitis group streptococci2021

    • Author(s)
      Matsumoto Airi、Tabata Atsushi、Ohkura Kazuto、Oda Hiroki、Kodama Chihiro、Ohkuni Hisashi、Takao Ayuko、Kikuchi Ken、Tomoyasu Toshifumi、Nagamune Hideaki
    • Journal Title

      Microbiology and Immunology

      Volume: 65 Issue: 2 Pages: 61-75

    • DOI

      10.1111/1348-0421.12868

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Isomerization of TX-2036 Derivatives on the Interaction With Tyrosine Kinase Domain of EGF Receptor2020

    • Author(s)
      OHKURA KAZUTO、TABATA ATSUSHI、UTO YOSHIHIRO、HORI HITOSHI
    • Journal Title

      Anticancer Research

      Volume: 40 Issue: 8 Pages: 4675-4680

    • DOI

      10.21873/anticanres.14466

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of isomerization of TX-2036 derivatives on the interaction with tyrosine kinase domain of EGF receptor.2020

    • Author(s)
      Ohkura K, Tabata A, Uto Y, Hori H.
    • Journal Title

      Anticancer Res.

      Volume: 印刷中

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Correlation Between Radiosensitizing Activity and the Stereo-structure of the TX-2036 Series of Molecules.2019

    • Author(s)
      Ohkura K, Tabata A, Uto Y, Hori H.
    • Journal Title

      Anticancer Res.

      Volume: 39 Issue: 8 Pages: 4479-4483

    • DOI

      10.21873/anticanres.13622

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro2019

    • Author(s)
      Tabata A, Yamada T, Ohtani H, Ohkura K, Tomoyasu T, Nagamune H.
    • Journal Title

      Journal of Oral Microbiology

      Volume: in press

    • NAID

      120006711364

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structure-associated Functional Control of TX-1877 Series by Glyco-conjugation.2018

    • Author(s)
      Ohkura K, Kawaguchi Y, Tatematsu Y, Tabata A, Uto Y, Hori H.
    • Journal Title

      Anticancer Res.

      Volume: 38 Issue: 7 Pages: 4241-4245

    • DOI

      10.21873/anticanres.12720

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] ミチス群レンサ球菌が産生するコレステロール依存性細胞溶解毒素モチーフを持つ細胞接着分子の特性2021

    • Author(s)
      松本愛理、田端厚之、大倉一人、児玉千紘、大國寿士、高尾亞由子、菊池賢、友安俊文、長宗秀明
    • Organizer
      第94回日本細菌学会総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] リアルタイム薬剤検出システムの構築試行:ミトコンドリア酸素消費速度を利用した担体からの薬剤放出特性の解析2020

    • Author(s)
      田端厚之、立松洋平、大倉一人
    • Organizer
      第24回バイオ治療法研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Chiral-2-nitroimidazole骨格を足場としたTX-2036誘導体の特性:EGF受容体キナーゼドメインとの相互作用2019

    • Author(s)
      田端厚之、宇都義浩、堀  均、大倉一人
    • Organizer
      第23回バイオ治療法研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] 脂肪酸結合タンパク質(FABP)のアイソフォーム間における薬物結合能の比較2019

    • Author(s)
      山本篤司、服部奈々美、江口昂佑、田口裕基、刀根淳貴、大倉一人
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Chiral-2-nitroimidazole骨格を有するTX-2036誘導体の開発:放射線増感能の修飾に関わる分子特性の検証2018

    • Author(s)
      田端厚之、宇都義浩、堀 均、大倉一人
    • Organizer
      第22回バイオ治療法研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] 薬剤キャリアのミセルが示す癌細胞障害性2018

    • Author(s)
      西平有里菜、田端厚之、友安俊文、大倉一人、長宗秀明
    • Organizer
      第22回バイオ治療法研究会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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