| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
At present, it is difficult to accurately predict the degree of tubular secretion involved in renal excretion of drugs and the contribution of drug transporters involved in tubular secretion. In this study, we examined the effect of expression of transcription factors involved in the regulation of drug transporter expression on the expression of drug transporters in human kidney-derived HK-2 cells. It was demonstrated that HK-2 cells stably expressing HNF1α showed a increased expression of megalin, which is responsible for endocytosis of low molecular weight proteins and drugs. The nephrotoxicity of aminoglycoside antibiotics, substrates of megalin, is significantly enhanced in HK-2 cells stably expressing HNF1α.
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