| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Sec6 is one of the subunits of the exocyst, an evolutionarily conserved eight-protein complex comprising Sec3 (EXOC1), Sec5 (EXOC2), Sec6 (EXOC3), Sec8 (EXOC4), Sec10 (EXOC5), Sec15 (EXOC6), Exo70 (EXOC7) and Exo84 (EXOC8) subunits. Recently, several researches have showed that Sec6 is associated with various cellular mechanisms such as neurite growth, cell adhesion, cell polarity, cell migration, cell cycle, NFκB signaling, and apoptosis. These functions may be dependent or independent on its basic role of Sec6 in exocytosis.
However, the relationship between Sec6 and PP2A in the Raf-MEK-ERK-p90RSK cascade is still largely unclear. We demonstrate that Sec6 regulates PP2A phosphatase activity through the modulation of the binding of PP2A-A and PP2Ac subunit, thereby modulating the phosphorylation of p90RSK at Thr359 and Ser380 and glycogen synthase kinase 3β at Ser9 and the expression of zinc finger E-box binding homeobox 1 (ZEB1), Vimentin, and zonula occludens 3 (ZO-3).
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