| Project/Area Number |
18K06832
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 48010:Anatomy-related
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| Research Institution | Ehime University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 成長版 / 組織系譜解析 / ヘッジホッグシグナル / 造血ニッチ / 骨髄 / Hhシグナル / 成長板 / 骨髄ニッチ / 造血幹細胞 / ヘッジホッグ / 遺伝子改変マウス |
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| Outline of Final Research Achievements |
Longitudinal bone growth progresses by continuous bone replacement of epiphyseal cartilaginous tissue, known as “growth plate”, produced by columnar proliferated- and differentiated-epiphyseal chondrocytes. The endochondral ossification process at the growth plate is governed by paracrine signals secreted from terminally differentiated chondrocytes (hypertrophic chondrocytes), and hedgehog signaling is one of the best known regulatory signaling pathways in this process. To investigate the developmental relationship between longitudinal endochondral bone formation and osteogenic progenitors under the influence of hedgehog signaling at the growth plate, genetic lineage tracing was carried out with the use of Gli1CreERT2 mice line. Our results show an evidence of the developmental contribution of endochondral progenitors under the influence of epiphyseal chondrocyte-derived secretory signals in bone marrow formation.
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| Academic Significance and Societal Importance of the Research Achievements |
骨髄は、白血病、多発性骨髄腫、再生不良性貧血などの難治性造血器疾患の罹患臓器であるが、これまで造血幹細胞を含む血液細胞を主体とした研究がなされてきた。しかし、近年の造血幹細胞を支持する非血液細胞(ニッチ)の同定により、造血器疾患における造血ニッチの関与が注目され始めている。本研究の遂行により得られた成果は、未だに未知の難治性造血器疾患の発症機序解明や造血ニッチを標的とする新しい視点からの治療法確立などに貢献する可能性があり、学術のみならず社会への波及効果も強く期待される。
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