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A new subpopulation of astrocytes that subserve GABAergic neural transmission in the brain.

Research Project

Project/Area Number 18K06836
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 48010:Anatomy-related
Research InstitutionNara Medical University

Principal Investigator

Wanaka Akio  奈良県立医科大学, 医学部, 教授 (90210989)

Co-Investigator(Kenkyū-buntansha) 竹村 晶子 (森田 晶子)  奈良県立医科大学, 医学部, 助教 (70647049)
田中 達英  奈良県立医科大学, 医学部, 講師 (80567032)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsアストロサイト / GABA / 抑制性神経伝達物質 / 淡蒼球 / 大脳基底核 / Olig2 / 転写因子 / GFAP / 抑制性神経伝達 / トランスポーター / レーザーマイクロダイセクション / シングル細胞遺伝子発現解析 / GABA伝達 / shRNA / 神経伝達
Outline of Final Research Achievements

We found Olig2-expressing astrocytes, which are distinct from GFAP-expressing astrocytes. Olig2-astrocytes are distributed widely in the adult brain. They are localized to the brain nuclei known to contain inhibitory neuronal terminals, suggesting that Olig2-astrocytes may be involved in the inhibitory GABAergic transmission as a component of "tripartite synapse". The globus pallidus (GPe) is one of the representative nucleus that contains a number of Olig2-astrocytes. Interestingly, Olig2-astrocytes and GFAP-astrocytes occupied mutually-exclusive teritories in the GPe. We found that the processes of Olig2-astrocytes were associated with GABAergic neuronal terminals. We tried to knock-down GAT-3, a glial GABA transporter, with adeno-associated virus vector harboring shRNA of GAT-3. The knock-down efficiency was unfortunately low and we did not see any effects on behaviors of virus vector-transduced mice.

Academic Significance and Societal Importance of the Research Achievements

アストロサイトは脳内で神経細胞の機能を補助する役割をするグリア細胞の一種である。これまでGFAPという蛋白がアストロサイトを特徴付けるという定説があったが、今回我々はGFAPが少なく、代わりにOlig2という転写因子を発現する新しいアストロサイト集団を見出した。この新しい集団は脳内に広く存在したが、特にGABAを伝達物質とする抑制性神経回路の近傍に存在する特徴があった。このような抑制性神経を補助するようなアストロサイトはこれまで報告されておらず、現在我々はこのアストロサイトの機能的意義を追求している。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (23 results)

All 2021 2020 2019 2018

All Journal Article (13 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 13 results,  Open Access: 8 results) Presentation (10 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] SNX25 regulates proinflammatory cytokine expression via the NF-κB signal in macrophages.2021

    • Author(s)
      Nishimura K, Tanaka T, Takemura S, Tatsumi K, Wanaka A.
    • Journal Title

      Plos One

      Volume: 16 Issue: 3 Pages: e0247840-e0247840

    • DOI

      10.1371/journal.pone.0247840

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Circadian rhythms of soting nexin 25 in the mouse suprachiasmatic nucleus2020

    • Author(s)
      Takemura S, Nagano M, Isonishi A, Tanaka T, Tatsumi K, Yamano M, Minami Y, Shigeyoshi Y, Wanaka A
    • Journal Title

      Neuroscience Letter

      Volume: 727 Pages: 134897-134897

    • DOI

      10.1016/j.neulet.2020.134897

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Depletion of microglia and macrophages with clodronate liposomes attenuates zymosan-induced Fos expression and hypothermia in the adult mouse.2020

    • Author(s)
      Takagi S, Murayama S, Torii K, Takemura-Morita S, Kurganov E, Nagaoka S, Wanaka A, Miyata S.
    • Journal Title

      J Neuroimmunol.

      Volume: 344 Pages: 577244-577244

    • DOI

      10.1016/j.jneuroim.2020.577244

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neural expression of sorting nexin 25 and its regulation of tyrosine receptor kinase B trafficking2020

    • Author(s)
      Shoko Takemura, Ayami Isonishi, Tatsuhide Tanaka, Hiroaki Okuda, Kouko Tatsumi, Mariko Yamano, Akio Wanaka
    • Journal Title

      Brain Structure and Function

      Volume: 225 Issue: 9 Pages: 2615-2642

    • DOI

      10.1007/s00429-020-02144-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo2019

    • Author(s)
      Shimomura T, Kawakami M, Tatsumi K, Tanaka T, Morita-Takemura S, Kirita T, Wanaka A
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 52 Issue: 1 Pages: 19-26

    • DOI

      10.1267/ahc.18038

    • NAID

      130007605401

    • ISSN
      0044-5991, 1347-5800
    • Year and Date
      2019-02-28
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice2019

    • Author(s)
      Ito Taeko、Tatsumi Kouko、Takimoto Yasumitsu、Nishimura Tadashi、Imai Takao、Yamanaka Toshiaki、Takeda Noriaki、Wanaka Akio、Kitahara Tadashi
    • Journal Title

      Brain Sciences

      Volume: 9 Issue: 11 Pages: 329-329

    • DOI

      10.3390/brainsci9110329

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Blood-to-brain communication in the hypothalamus for energy intake regulation.2019

    • Author(s)
      Morita-Takemura S, Wanaka A.
    • Journal Title

      Neurochem Int.

      Volume: 128 Pages: 135-142

    • DOI

      10.1016/j.neuint.2019.04.007

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Responses of perivascular macrophages to circulating lipopolysaccharides in the subfornical organ with special reference to endotoxin tolerance.2019

    • Author(s)
      Morita-Takemura S, Nakahara K, Hasegawa-Ishii S, Isonishi A, Tatsumi K, Okuda H, Tanaka T, Kitabatake M, Ito T, Wanaka A.
    • Journal Title

      J Neuroinflammation

      Volume: 16 Issue: 1 Pages: 39-51

    • DOI

      10.1186/s12974-019-1431-6

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Abrogated Caveolin-1 expression via histone modification enzyme Setdb2 regulates brain edema in a mouse model of influenza-associated encephalopathy.2019

    • Author(s)
      Imakita N, Kitabatake M, Ouji-Sageshima N, Hara A, Morita-Takemura S, Kasahara K, Matsukawa A, Wanaka A, Mikasa K, Ito T.
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 284-284

    • DOI

      10.1038/s41598-018-36489-8

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Olig2-Lineage Astrocytes: A Distinct Subtype of Astrocytes That Differs from GFAP Astrocytes2018

    • Author(s)
      Tatsumi Kouko、Isonishi Ayami、Yamasaki Miwako、Kawabe Yoshie、Morita-Takemura Shoko、Nakahara Kazuki、Terada Yuki、Shinjo Takeaki、Okuda Hiroaki、Tanaka Tatsuhide、Wanaka Akio
    • Journal Title

      Front. Neuroanat.

      Volume: 12 Pages: 1-17

    • DOI

      10.3389/fnana.2018.00008

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Propofol induces nuclear localization of Nrf2 under conditions of oxidative stress in cardiac H9c2 cells2018

    • Author(s)
      Shinjo Takeaki、Tanaka Tatsuhide、Okuda Hiroaki、Kawaguchi Akira T.、Oh-hashi Kentaro、Terada Yuki、Isonishi Ayami、Morita-Takemura Shoko、Tatsumi Kouko、Kawaguchi Masahiko、Wanaka Akio
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 4 Pages: e0196191-e0196191

    • DOI

      10.1371/journal.pone.0196191

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] NGF and BDNF expression in mouse DRG after spared nerve injury2018

    • Author(s)
      Terada Y, Morita-Takemura S, Isonishi A, Tanaka T, Okuda H, Tatsumi K, Shinjo T, Kawaguchi M, Wanaka A
    • Journal Title

      Neurosci Lett.

      Volume: 686 Pages: 67-73

    • DOI

      10.1016/j.neulet.2018.08.051

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The inner mitochondrial membrane protein ANT1 modulates IL-6 expression via the JNK pathway in macrophages2018

    • Author(s)
      Nakahara K, Tanaka T, Okuda H, Isonishi A, Morita-Takemura S, Tatsumi K, Wanaka A
    • Journal Title

      FEBS Lett.

      Volume: 592 Issue: 22 Pages: 3750-3758

    • DOI

      10.1002/1873-3468.13269

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Olig2由来アストロサイトはアミノ酸トランスポーターSLC7A10を発現する2021

    • Author(s)
      辰巳晃子、絹川薫、石西綾美、奥田洋明、竹村晶子、田中達英、森英一郎、和中明生
    • Organizer
      第126回日本解剖学会総会・全国学術総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Olig2アストロサイトの抑制性シナプスとの関与2020

    • Author(s)
      辰巳晃子、石西綾美、竹村晶子、田中達英、和中明生
    • Organizer
      第125回日本解剖学会総会(山口)
    • Related Report
      2019 Research-status Report
  • [Presentation] Olig2アストロサイトの脳内分布と抑制性シナプスとの関与2019

    • Author(s)
      辰巳晃子、石西綾美、山崎美和子、河邉良枝、中原一貴、奥田洋明、田中達英、和中明生
    • Organizer
      第124回日本解剖学会総会(新潟)
    • Related Report
      2019 Research-status Report
  • [Presentation] Comparison of molecular signatures of Olig2-lineage astrocyte and GFAP-astrocytes in the globus pallidus using laser micro-dissection2019

    • Author(s)
      辰巳晃子、石西綾美、竹村晶子、田中達英、和中明生
    • Organizer
      Neuro2019 (新潟)
    • Related Report
      2019 Research-status Report
  • [Presentation] Comparison of molecular signatures of Olig2-lineage astrocyte and GFAP-astrocyte using laser microdissection2019

    • Author(s)
      辰巳晃子、石西綾美、竹村晶子、田中達英、和中明生
    • Organizer
      ISN-ASN 2019 meeting (Montreal, Canada)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 感知系脳室周囲器官の血管周囲環境と炎症情報伝達2018

    • Author(s)
      森田-竹村晶子1、中原一貴1、長谷川-石井さなえ2、石西綾美1、辰巳晃子1、奥田洋明3、田中達英1、和中明生1
    • Organizer
      第123回日本解剖学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] FACS解析による成熟脳に存在するOlig2系譜アストロサイトの分離2018

    • Author(s)
      辰巳晃子、石西綾美、河邉良枝、森田-竹村晶子、中原一貴、田中達英、和中明生
    • Organizer
      第123回日本解剖学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] Multiplex staining法を用いた脳梁内数珠状グリア細胞の免疫組織化学的解析2018

    • Author(s)
      河邉 良枝、田中 達英、森田-竹村 晶子、中原 一貴、辰巳 晃子、和中 明生
    • Organizer
      第123回日本解剖学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] 血中のLPSを脳で感知する:脳弓下器官血管周囲腔マクロファージの役割2018

    • Author(s)
      森田-竹村晶子、中原一貴、長谷川ー石井さなえ、石西綾美、辰巳晃子、奥田洋明、田中達英、和中明生
    • Organizer
      第41回日本神経科学学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ANT1 modulates IL-6 expression via the JNK pathway in macrophages2018

    • Author(s)
      中原 一貴1、田中 達英1、奥田 洋明2、石西 綾美1、森田-竹村 晶子1、辰巳 晃子1、和中 明生1
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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